Effects of miR-488-3p on renal tubular epithelial cell injury by regulating the cGAS-STING signaling pathway
10.12007/j.issn.0258-4646.2025.11.004
- VernacularTitle:miR-488-3p调控cGAS-STING信号通路对肾小管上皮细胞损伤的影响
- Author:
Fangli XU
1
;
Huan WANG
1
;
Ke XU
1
Author Information
1. 新乡市中心医院肾内科,河南新乡 453000
- Publication Type:Journal Article
- Keywords:
miR-488-3p;
renal tubular epithelial cell;
acute kidney injury;
apoptosis;
oxidative stress
- From:
Journal of China Medical University
2025;54(11):982-987
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-488-3p on renal tubular epithelial cell injury by regulating the cyclic guanosine monophosphate-adenosine monophosphate synthase(cGAS)-stimulator of interferon gene(STING)signaling pathway.Methods Human renal tubular epithelial HK-2 cells were divided into control,hypoxia-reoxygenation(HR),inhibition control,miR-488-3p inhibition,cGAS-STING pathway activator(RocA),and miR-488-3p inhibition+RocA groups.Quantitative real-time polymerase chain reaction was used to detect miR-488-3p expression in HK-2 cells.Cell viability was assessed using the CCK-8 assay.Enzyme-linked immunosorbent assay was used to measure the levels of interleukin(IL)-10,IL-1β,and tumor necrosis factor-α(TNF-α)in the culture supernatant of HK-2 cells.The DCFH-DA method was applied to detect the average fluorescence intensity of reactive oxygen species(ROS)in cells.Results Compared with the control group,the HR group showed increased miR-488-3p expression,IL-1β and TNF-α levels,average fluorescence intensity of ROS,malondialdehyde(MDA)levels,apoptosis rate,and protein expression of cleaved caspase-3,Bax,cGAS,and STING,whereas cell viability,IL-10 levels,and superoxide dismutase(SOD)activity were reduced in the HR group(P＜0.05).Com-pared with the HR and inhibition control groups,the miR-488-3p inhibition group exhibited decreased expression of miR-488-3p,IL-1β and TNF-α levels,average fluorescence intensity of ROS,MDA levels,apoptosis rate,and protein levels of cleaved caspase-3,Bax,cGAS,and STING,whereas cell viability,IL-10 levels,and SOD activity were increased(P＜0.05).Conclusion Downregulation of miR-488-3p expression mitigates HR-induced inflammation,oxidative stress,and apoptosis in HK-2 cells,thereby reducing cell damage.The under-lying mechanisms may be associated with an inhibition of the cGAS-STING signaling pathway.
