Effect of miR-92a-3p on the progression of ischemic stroke via SIRT1 suppression
10.12007/j.issn.0258-4646.2025.11.003
- VernacularTitle:miR-92a-3p通过抑制SIRT1参与缺血性脑卒中的发生
- Author:
Juan PANG
1
;
Wei YANG
;
Junru YIN
;
Fenqing SHANG
Author Information
1. 西北大学附属胸科医院转化医学中心,西安 710100
- Publication Type:Journal Article
- Keywords:
miR-92a-3p;
silent information regulator 1;
ischemic stroke
- From:
Journal of China Medical University
2025;54(11):977-981
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the molecular mechanisms of action of miR-92a-3p in ischemic stroke(IS).Methods Database analysis was performed to evaluate miR-92a-3p expression in patients with IS.Oxygen-glucose deprivation/reoxygenation(OGD/R)of HT22 hippocampal neurons was used as an in vitro IS model.PCR was performed to analyze miR-92a-3p expression in HT22 cells fol-lowing 2,4,6,and 8 h of OGD/R.Western blotting was used to analyze the protein expression levels of silent information regulator 1(SIRT1),an miR-92a-3p target gene,and the apoptosis-related protein caspase-3 in HT22 cells after 4 h of OGD/R.Middle cerebral artery occlusion(MCAO)was used as an animal model of IS.Western blotting and PCR were used to analyze the mRNA and protein expression levels of SIRT1 and caspase-3 in the brain tissues of mice following MCAO.Results Database analysis showed increased miR-92a-3p expression in fetal rat cortical neurons after 12 h of OGD and in the brain tissues of mice 3 days post-MCAO.Following 2,4,6,and 8 h of OGD/R in HT22 cells,the expression of miR-92a-3p increased,accompanied by decreased expression of SIRT1 and increased expres-sion of caspase-3.In the brain tissues of mice model of IS,SIRT1 expression decreased and caspase-3 expression increased.Conclusion miR-92a-3p is involved in IS by promoting neuronal apoptosis via SIRT1 inhibition.
