Effect of Cardamonin on Proliferation,Migration and Invasion of CAL27 Cells in Tongue Squamous Cell Carcinoma
10.11969/j.issn.1673-548X.2025.02.021
- VernacularTitle:小豆蔻明对舌鳞状细胞癌CAL27细胞增殖、迁移和侵袭的影响
- Author:
Wenrui JIANG
1
;
Jianhui LIN
1
;
Rui HAN
1
Author Information
1. 233000 蚌埠医科大学第一附属医院口腔科
- Publication Type:Journal Article
- Keywords:
Tongue squamous cell carcinoma;
CAL27 cell;
Cardamonin;
Migration;
Invasion;
Epithelial-mesenchymal transition;
PI3K/Akt signaling pathway
- From:
Journal of Medical Research
2025;54(2):132-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Cardamonin on the proliferation,migration and invasion of CAL27 cells in tongue squamous cell carcinoma,and to elucidate its possible mechanism of action.Methods CAL27 cells were treated with different concentra-tions of Cardamonin for 24h and 48h respectively.CCK-8 assay was used to detect cell proliferation,and the optimal drug concentration and action time were screened for subsequent experiments.Clonal formation assay was used to detect cell proliferation.Scratch test and Transwell test were used to detecte the ability of cell migration and invasion.Western blot method was used to detecte the expression levels of EMT related protein and phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)pathway related protein.Results Within a cer-tain concentration,Cardamonin inhibited the proliferation,migration and invasion of tongue squamous cell carcinoma CAL27 cells in a concentration-dependent manner,and the inhibitory effect became more obvious with the increase of concentration and time in a certain range.In addition,with the increase of drug concentration,the protein expression of E-cadherin showed an upward trend,while the pro-tein expression of N-cadherin,Vimentin and phosphorylated efferent molecules downstream of PI3K(p-PI3K and p-Akt)showed a downward trend.Treatment with PI3K agonist 740Y-P could partially reverse the anticancer effect of Cardamonin,and the differences were statistically significant(P＜0.05,P＜0.01).Conclusion Cardamonin can inhibit the proliferation,migration and invasion of CAL27 cells in tongue squamous cell carcinoma cells,and its mechanism may be related to the regulation of EMT process by inhibiting PI3K/Akt signaling pathway,which provides a new potential strategy and drug target for the treatment of tongue squamous cell carcinoma.
