Efficacy of targeted next-generation sequencing in the rapid detection of Mycobacterium
10.3969/j.issn.1002-2694.2025.00.162
- VernacularTitle:靶向高通量测序在快速检测分枝杆菌中的应用
- Author:
Daichen JU
1
;
Jiamin LIN
;
Hua CHEN
;
Pinru CHEN
;
Biyi SU
;
Yaoju TAN
;
Jinxing HU
;
Jialou ZHU
Author Information
1. 呼吸疾病全国重点实验室,广州市结核病研究重点实验室,广州市胸科医院检验科,广州医科大学结核病防治研究院,广州 510095
- Publication Type:Journal Article
- Keywords:
targeted next-generation sequencing;
Mycobacterium;
non-tuberculous mycobacteria;
Mycobacterium tuberculosis;
culture identification method
- From:
Chinese Journal of Zoonoses
2025;41(10):1016-1024
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to evaluate the application value of targeted next-generation sequencing(tNGS)for rapid detection of Mycobacterium tuberculosis.From September 2021 to October 2024,1 178 clinical samples from hospitalized pa-tients at the Non-tuberculous Mycobacteria Disease Diagnosis and Treatment Center of Guangzhou Chest Hospital were collected,in-cluding 272 sputum samples,871 broncho alveolar lavage fluid(BALF)samples,and 35 other samples.These samples were analyzed with tNGS and the liquid culture+DNA microarray chip method(referred to as the"culture identification method"),and the detection results between methods were compared.The numbers of Mycobacterium tuberculosis complex(MTBC)samples identified with tNGS and the culture identification method were 172 and 127,respectively,and the numbers of non-tuberculous mycobacteria(NTM)samples detected were 517 and 474,respectively.The detection rate of Mycobacterium was 56.88%with tNGS and was significantly lower(49.49%)with the culture identification method(χ2=13.27,P<0.05).For NTM identification,when sputum was used,the detection rate of tNGS was higher than that of the culture identification method(χ2=24.14,P<0.05).However,when BALF was used,no significant difference in detection rates was observed between tNGS and the culture identification method(χ2=3.97,P=0.06).When different sample types from the same patient were analyzed with tNGS for NTM,BALF was found to be a better choice than sputum(χ2=4.05,P<0.05).However,with the culture identification method,we observed no significant difference between sample types(χ2=2.72,P=0.10).Furthermore,both the MTBC and NTM sequences detected with tNGS were significantly higher in the culture-positive group than the culture-negative group.With increasing sequence numbers,the proportion of cases in the culture-positive group progressively rose,whereas an inverse trend was observed in the culture-negative group.With clinical diagno-sis as the reference standard,the sensitivity,specificity,and Kappa value of tNGS and the culture identification method in identifying mycobacterial diseases were 85.41%,91.32%,0.74 and 74.73%,93.15%,0.63,respectively.Compared with the culture identifica-tion method,tNGS for rapid detection of Mycobacterium exhibited excellent sensitivity,specificity and consistency,and its timeliness should help clinicians make precise individualized treatment plans earlier.
