Effects of ncBCG201 on BCG stress adaptation and intracellular survival
10.3969/j.issn.1002-2694.2025.00.161
- VernacularTitle:ncBCG201对BCG适应逆境及胞内生存能力的影响
- Author:
Zejin DU
1
;
Kailun ZHANG
;
Ling FAN
;
Han LIU
;
Aizhen GUO
;
Yingyu CHEN
Author Information
1. 农业微生物资源发掘与利用全国重点实验室,动物科技学院动物医学院,华中农业大学,武汉 430070
- Publication Type:Journal Article
- Keywords:
Bacillus Calmette Guerin(BCG);
Mycobacterium tuberculosis(M.tuberculosis);
sRNA;
ncBCG201;
stress adapta-tion
- From:
Chinese Journal of Zoonoses
2025;41(10):1011-1015
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the effect of small RNA ncBCG201 on the stress adaptation and intracellular survival of Myco-bacterium bovis.Differentially expressed ncRNAs were screened with RNA-seq after BCG infection of THP-1 macrophages.The ncBCG201 overexpression strain was constructed,and its stress survival,growth,biofilm formation,and intracellular survival were assessed.The ncBCG201 strain showed slower growth and earlier entry into stationary phase.Survival significantly increased under car-bon starvation but decreased under membrane pressure.Biofilm formation capability decreased.Intracellular survival in THP-1 macro-phages at 24 h and 48 h was significantly higher than observed in the control.Therefore,ncBCG204 enhances BCG survival within macrophages by modulating stress adaptability.
