Relationship and Potential Mechanisms between MiRNA and Ulcerative Colitis from Meta-analysis of Mendelian Randomization
10.11969/j.issn.1673-548X.2025.02.015
- VernacularTitle:孟德尔随机化联合Meta分析miRNA在溃疡性结肠炎中的关系及潜在机制
- Author:
Xuyong CHEN
1
;
Xiaohong YANG
;
Haidong WU
Author Information
1. 570100 海口,海南省人民医院/海南医科大学附属海南医院
- Publication Type:Journal Article
- Keywords:
Ulcerative colitis;
Mendelian randomization;
MicroRNA;
Meta-analysis;
MAP3K1
- From:
Journal of Medical Research
2025;54(2):88-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective Mendelian randomization(MR)combined with Meta-analysis(MR-Meta analysis)and bioinformatics mining were used to identify the causal relationship between microRNA(miRNA)and ulcerative colitis(UC).The potential biological functions and pathways were analyzed to search for new biomarkers and potential therapeutic targets.Methods MiRNA exposure data and multiple UC outcome data sets were used to identify the causal relationship between miRNA and UC by MR-meta analysis.Then,the mi-croarray data of UC was extracted by gene expression database for differential analysis,the differential expression of MR-Meta-miRNAs was detected,and the differential target genes of the detected candidate miRNA were screened by machine learning algorithm for key genes.Then,the function and pathway of key genes were analyzed by immune cell infiltration and single gene enrichment analysis(GSEA),and the ceRNA regulatory network was constructed.Results Nine miRNA were identified by MR-Meta analysis and were significantly associated with UC risk.The GES48959 chip data analysis showed that the hsa-miR-196b-5p was significantly low-ex-pressed in UC tissues,the differential genes targeted by hsa-miR-196b-5p were then used to obtain the key gene MAP3K1 by two ma-chine learning algorithms.GSEA and immune-infiltration analysis showed that MAP3K1 was a key gene in regulating immune inflamma-tion.The ceRNA regulatory network indicates that lncRNA/hsa-miR-196b-5p/MAP3K1had the potential to regulate UC lesions.Conclusion There is a causal relationship between miRNA and UC,among which hsa-miR-196b-5p is closely related to the risk of UC.The hsa-miR-196b-5p/MAP3KI pathway may affect the occurrence and progression of diseases as a molecular mechanism regu-lating UC immune inflammation,and provide a new idea for exploring the effective diagnosis and treatment of UC in the future.
