Effect of Silencing BHLHE41 on Angiotensin Ⅱ-induced Hypertrophy and Apoptosis of H9C2 Cardiomyocytes
10.11969/j.issn.1673-548X.2025.02.012
- VernacularTitle:沉默BHLHE41对血管紧张素Ⅱ诱导的H9C2心肌细胞肥大和凋亡的影响
- Author:
Luqing XU
1
;
Na YU
1
;
Kebing JIANG
1
Author Information
1. 430060 武汉大学人民医院心血管内科、代谢与相关慢病湖北省重点实验室
- Publication Type:Journal Article
- Keywords:
Cardiomyocytes;
Hypertrophy;
Apoptosis;
BHLHE41;
Rats
- From:
Journal of Medical Research
2025;54(2):70-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of basic helix-loop-helix family member e41(BHLHE41)on angiotensin Ⅱ(Ang Ⅱ)-induced hypertrophy and apoptosis of H9C2 cardiomyocytes.Methods The expression level of BHLHE41 stimulated by Ang Ⅱ was investigated,and the cells were divided into normal group and Ang Ⅱ group.To investigate the effect of silencing BHLHE41(siBHLHE41)on cardiomyocyte hypertrophy and apoptosis,the cells were divided into 4groups:blank group[transfected with small in-terfering RNA(siRNA)+phosphate buffered saline(PBS)],siBHLHE41 group[transfected with siBHLHE41+PBS],model group(transfected with siRNA+Ang Ⅱ)and experimental group(transfected with siBHLHE41+Ang Ⅱ).The mRNA levels of BHLHE41,β-myosin heavy chain(β-MHC),atrial natriuretic peptide(ANP),B-type natriuretic peptide(BNP),poly ADP-ribose polymer-ase(PARP),B-cell lymphoma-2(Bcl-2),B-cell lymphoma-2-associated X protein(Bax),and p53 were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).The protein expression levels of BHLHE41,Bax,Bcl-2,PARP and p53 were detected by Western blot.Phalloidin staining was used to observe the surface area of cardiomyocytes,TUNEL staining was used to observe cardiomyocytes apoptosis.Results Compared with the normal group,the mRNA and protein expression levels of BHL-HE41 was higher in the Ang Ⅱ group.Compared with blank group,the mRNA expression levels of β-MHC,ANP,BNP in the model group were upregulated,and the protein and mRNA expression levels of PARP,Bax and p53 in model group were significantly increased,the expression level of Bcl-2 was significantly decreased,the surface area of cardiomyocytes was significantly increased,and the apopto-sis rate of cardiomyocytes was significantly increased(P＜0.05).Compared with model group,the mRNA expression levels of β-MHC,ANP and BNP in the experimental group were upregulated,and the protein and mRNA expression levels of PARP,Bax and p53 in experi-mental group were significantly increased,the expression level of Bcl-2 was significantly decreased,the surface area of cardiomyocytes was increased,and the apoptosis rate of cardiomyocytes was significantly increased(P＜0.05).Conclusion Silencing BHLHE41 can aggravate Ang Ⅱ-induced hypertrophy and apoptosis of H9C2 cardiomyocytes,which may be related to the p53 activation of the apoptotic signaling pathway.
