Effect of Electrical Stimulation on Healing of Infected Wound in Rats with Infected Pressure Injury
10.11969/j.issn.1673-548X.2025.02.008
- VernacularTitle:电刺激对压力性损伤大鼠感染创面愈合的作用
- Author:
Linyan YUAN
1
;
Menghang WU
;
Xue YANG
Author Information
1. 610041 成都,四川大学华西医院肝脏外科
- Publication Type:Journal Article
- Keywords:
Electrical stimulation;
Pressure injury;
Pressure sore;
Infected wound;
Rats
- From:
Journal of Medical Research
2025;54(2):43-47,87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the healing effect of electrical stimulation in the treatment of infected pressure injury in rats.Methods Twenty-four male SD rats were divided into blank group,model group and electrical stimulation group by random number ta-ble method.The infected pressure injury model of stage 3 was established by magnet external compression and observed for 2 weeks.The model group healed naturally,and the electrical stimulation group underwent electroacupuncture.The wound areas of rats were measured on the 1st,3rd,5th,7th,10th and 14th days after modeling,the wound healing rate was calculated,and the experimental wound assess-ment tool(EWAT)scores on the 3rd,7th and 14th days were evaluated.On the 7th and 14th days,hematoxylin-eosin staining and Masson staining were used to observe the pathological changes of wound skin tissue,and the expression levels of inducible nitric oxide syn-thase(INOS),CD206,CD31 andα-smooth muscle actin(α-SMA)antibodies were measured by fluorescent staining.Results The wound healing rate of the electrical stimulation group were higher than that in the model group at the 3rd,5th,7th,10th and 14th days of treatment,and the difference were statistically significant(P＜0.05).The EWAT scores of wounds in the electrical stimulation group were lower than that in the model group at the 3rd,7th and 14th days,and the difference were statistically significant(P＜0.05).Tissue morphology observation showed that electrical stimulation reduced wound inflammation,promoted capillary production and collagen deposi-tion,and accelerated epithelial crawling.Compared with the model group,electrical stimulation treatment reduced the expression level of INOS antibody and increased the expression levels of CD206 antibody,α-SMA antibody and CD31 antibody,and the difference was sta-tistically significant(P＜0.05).Conclusion Electrical stimulation can effectively promote the healing of infected wounds in rats with stage 3 pressure injury,promotes macrophage polarization and vascular reconstruction.
