Pterostilbene alleviates the neuroinflammation of cerebral ischemia/reperfusion injury in rats by regulating COX-2/PGD2/DPS pathway
10.16557/j.cnki.1000-7547.2024.06.013
- VernacularTitle:紫檀芪通过COX-2/PGD2/DPs通路减轻脑缺血/再灌注损伤大鼠的神经炎症
- Author:
Yingchun YANG
1
;
Xiaoliang ZHANG
1
;
Saihong GAO
1
;
Shuyu JIA
1
;
Jinrui WANG
1
;
Jibo WEI
1
;
Xiyue WANG
1
Author Information
1. 山西医科大学汾阳学院解剖学教研室,汾阳 032200
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion injury(CIRI);
pterostilbene(PTE);
COX-2/PGD2/DP pathway;
neu-roinflammation;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(6):761-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of pterostilbene(PTE)in preventing and treating neuroinflamma-tion after cerebral ischemia-reperfusion injury(CIRI)in rats.Methods:Ninety male SD rats were randomly divided in-to a sham group,a model group(MCAO/R),a low-dose PTE group(PTE-L),a medium-dose PTE group(PTE-M),and a high-dose PTE group(PTE-H).CIRI model was prepared by middle cerebral artery occlusion reperfusion(MCAO/R)in rats.The neurological deficit in rats was evaluated by Zea Longa score.The volume of cerebral infarc-tion was detected by TTC staining.The morphological changes of ischemic cortex was observed HE staining.The ex-pressions of cyclooxygenase-2(COX-2),prostaglandin D2 receptor(DP2)and prostaglandin D1 receptor(DP1)were detected by RT-qPCR and Western Blot.The expressions of prostaglandin D2(PGD2),interleukin-1 β(IL-1β)and tumor necrosis factor-α(TNF-α)were detected by ELISA.Results:Compared with the sham group,the MCAO/R group showed a significant increase in neurological scores(P＜0.05),a significant increase in cerebral infarction vol-ume(P＜0.05),and aggravated cortical damage in the ischemic area.Additionally,there were significant increase in the expressions of COX-2,DP2 mRNA and protein(P＜0.05),along with increased expressions of PGD2,IL-1β and TNF-α(P＜0.05).Compared with the MCAO/R group,the PTE-L,PTE-M,and PTE-H groups showed a significant decrease in neurological scores(P＜0.05),a significant decrease in cerebral infarction volume(P＜0.05),and markedly alleviated cortical damage in the ischemic region.Additionally,there were significant decrease in the expres-sions of COX-2,DP2 mRNA and protein(P＜0.05),along with decreased expressions of PGD2,IL-1β and TNF-α(P＜0.05).Furthermore,a dose-effect relationship was observed for the neuroprotective effects of PTE on brain tissue(P＜0.05).However,there were no significant differences in the expressions of DP,mRNA and protein among all groups(P＞0.05).Conclusion:PTE can attenuate the neuroinflammation of CIRI in rats by inhibiting COX-2/PGD2/DP2 signaling pathway.
