Stellate ganglion block alleviates inflammatory response in rats with acute peritonitis via TLR4/NF-κB pathway
10.16557/j.cnki.1000-7547.2024.06.012
- VernacularTitle:星状神经节阻滞通过TLR4/NF-κB通路缓解急性腹膜炎大鼠炎症反应
- Author:
Peng YANG
1
;
Yu CAO
1
;
Luwei JIN
1
;
Lin LI
1
;
Xuejun ZOU
1
Author Information
1. 三峡大学附属仁和医院麻醉科,宜昌 443001
- Publication Type:Journal Article
- Keywords:
stellate ganglion block(SGB);
acute peritonitis(AP);
inflammatory response;
TLR4/NF-κB path-way;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(6):754-760
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of stellate ganglion block(SGB)on serum inflammatory cytokines and the expression of toll-like receptor 4(TLR4),cysteine aspartate protease 3(caspase-3),and NF-κB(p65)pro-teins in rats with acute peritonitis(AP),with the goal of elucidating the mechanisms through which SGB modulates inflammatory responses.Methods:40 SPF grade male rats were randomly assigned to 4 groups:control group(Con-trol),acute peritonitis group(AP),acute peritonitis+right stellate ganglion block group(AP+SGB),and acute peri-tonitis+right stellate ganglion block+TLR4 inhibitor resatorvid group(AP+SGB+TAK-242),with 10 rats in each group.Acute peritonitis was induced in the AP,AP+SGB,and AP+SGB+TAK-242 groups via intraperitoneal injec-tion of 1 ml/100 g of 2％acetic acid solution.After establishing the model,blood samples were collected from the tail vein at 1 h,6 h,12 h,and 24 h respectively.Serum levels of interleukin-10(IL-10),tumor necrosis factor-alpha(TNF-α),interleukin-1 β(IL-1 β),and high mobility group protein B1(HMGB1)were measured using ELISA.After 24 hours,the peritoneal tissues were collected for analysis.Protein levels of TLR4,caspase-3,and NF-κB(p65)were quantified via Western Blot,while simultaneously,their mRNA expression levels were assessed using RT-qPCR.His-topathological alterations were evaluated by hematoxylin and eosin(HE)staining and then observed under optical microscopy.Results:At all time points,the AP,AP+SGB,and AP+SGB+TAK-242 groups showed significantly higher levels of IL-10,TNF-α,IL-1 β,and HMGB1 compared to the S group(P＜0.05).The AP+SGB group exhibi-ted significantly lower TNF-α,IL-1 β,and HMGB1 levels and higher IL-10 levels than the AP group at corresponding time points(P＜0.05).The AP+SGB+TLR4 group demonstrated further reductions in TNF-α,IL-1 β,and HMGB1 levels and additional increases in IL-10 levels relative to the AP+SGB group(P＜0.05).Both the AP+SGB and AP+SGB+TAK-242 groups showed significant decreases in TLR4,caspase-3,and NF-κB(p65)protein expression(P＜0.05).Similarly,TLR4 and NF-κB(p65)mRNA expression levels were markedly downregulated in these groups(P＜0.05).Conclusion:SGB can mitigate the inflammatory response in acute peritonitis rats by inhibiting the TLR4/NF-κB signaling pathway through the downregulation of TLR4 and NF-κB(p65).
