Modification of the device and fixation method for lateral ventricular catheterization in the rat
10.16557/j.cnki.1000-7547.2024.06.010
- VernacularTitle:大鼠侧脑室置管装置和置管固定方法的改良
- Author:
Long HE
1
;
Huaguo WU
;
Shuwei LIU
;
Zelong LI
;
Ying ZHU
;
Lianxiang ZHANG
Author Information
1. 宁夏医科大学颅脑疾病重点实验室 银川 750004
- Publication Type:Journal Article
- Keywords:
lateral ventricle;
catheterization device;
the fixation method of the catheter;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(6):739-746
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve a device and fixation method for ventricular catheterization in rats,which is mini-mally invasive,has a short stress period,and allows delayed drug administration or cerebrospinal fluid collection.Methods:Healthy adult male SD rats were divided into a Regular method group,Modified method group and Control group.In the Regular method group,commercially available cannula needles with internal cores were used for catheter-ization,fixed to the skull using dental cement along with fixing screws;in Modified method group an improved catheter-ization device was used along with its corresponding fixation technique;no treatment was given in Control group.Senso-ry function and motor function of rats were assessed by using hot plate test and footprint analysis respectively at 4 and 7 days after insertion;HE staining was used to observe the damage of brain tissue around the needle tract at 7 days of catheterization,and immunofluorescence staining was used to detect change in Iba-1 expression near the catheter needle tract.Results:The sensory and motor function impairments in the Modified method group were milder than those in the Regular method group(P＜0.05);HE staining showed that the needle tract in the Modified method group was signifi-cantly narrower than that in the Regular method group;immunofluorescence staining revealed a significantly higher num-ber of Iba-1 positive cells near the needle tract in the Regular method group compared to the Modified method group(P＜0.05).Conclusion:The modified catheterization device and fixation method described in this article result in minimal brain injury,shorter stress period,and can be used for delayed intraventricular drug administration or cerebro-spinal fluid collection.
