Oxytocin promotes arousal and cognitive function recovery in mice after anesthesia
10.16557/j.cnki.1000-7547.2024.06.005
- VernacularTitle:催产素促进小鼠麻醉后觉醒和认知功能的恢复
- Author:
Wenrui JIANG
1
;
Yuhan ZHOU
;
Hongyi JIA
;
Yiyao LI
;
Fang ZHOU
;
Fujuan MA
;
Yujie LIU
;
Mingming ZHANG
Author Information
1. 空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安 710032
- Publication Type:Journal Article
- Keywords:
locus ceruleus(LC);
oxytocin(OXT);
anesthesia;
arousal;
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(6):695-702
- CountryChina
- Language:Chinese
-
Abstract:
Objective:By observing the loss(LORR)and recovery of righting reflex(RORR)in mice after anes-thesia and the exploration of Y-maze,explore the role of oxytocin(OXT)in promoting arousal in mice.Methods:The expression of oxytocin receptor(OXTR)in the cell bodies and tyrosine hydroxylase(TH)positive immunoreactive neu-rons in locus coeruleus(LC)was verified by immunofluorescence staining.Using the function of intraperitoneal injec-tion anesthesia and gas anesthesia,and intraperitoneal injection,intrathecal injection,and right LC single tube catheter administration,OXT or saline given as experimental group and control group,respectively,explored the time needed in the induction anesthesia period and anesthesia recovery period of mice after administration,and conducted Y-maze test on groups with statistically different anesthesia results,then explored the exploration of novel arm in Y-maze after 6,9,18 and 24 hours of RORR.Results:In LC,TH and OXTR positive neurons had double labeling.There was no differ-ence in the time of RORR after intraperitoneal injection and intrathecal injection of OXT and saline respectively,and no difference in the time of LORR and RORR after single-tube cannula administration of OXT and saline with intraperitone-al anesthesia and gas anesthesia.Compared with the control group,the recovery time of mice in the experimental group under gas anesthesia given OXT with single cannula was reduced(P＜0.05).In this group,the exploration activities of Y-maze novel arm increased after 9 hours of anesthesia recovery(P＜0.05),but there was no significant statistical difference in the exploration activities of Y-maze novel arm at 6,18 and 24 hours.Western Blot showed that compared with the control group,the expression of TH in LC in the experimental group increased significantly within 9 hours after anesthesia(P＜0.05),and then began to decrease after 9 hours(P＜0.01).Conclusion:OXT can promote the arousal of mice,and the cognitive degree of mice given OXT recovered better after waking up for 9 hours after anesthe-sia.
