Exercise fatigue triggers depressive-like behaviour in rats via substance P-induced microglia activation
10.16557/j.cnki.1000-7547.2024.06.004
- VernacularTitle:运动疲劳通过P物质诱导的小胶质细胞活化引发大鼠抑郁样行为
- Author:
Chang SU
1
;
Zixuan XU
;
Shuo JIN
;
Qianyun YANG
;
Chao XI
;
Lina SUN
Author Information
1. 北京师范大学体育与运动学院,北京 100875
- Publication Type:Journal Article
- Keywords:
exercise fatigue;
substance P(SP);
microglia(MG);
p38 mitogen-activated protein kinase(p38 MAPK);
depression-like behaviour;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(6):688-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the central regulatory mechanisms underlying depression-like behavior induced by exercise fatigue in rats.Methods:Male Sprague-Dawley(SD)rats were randomly divided into a control group and a fatigue group.The exercise-induced fatigue model was established by a three-level incremental load treadmill training protocol.The sucrose preference test(SPT)was conducted to evaluate depressive-like behavior in the rats.Following the behavioral testing,Immunohistochemical staining was used to observe the expression of substance P(SP)and the activation level of microglia(MG)in the CA1 region of the hippocampus.Additionally,Western Blot were performed to observe the hippocampal phosphorylation level of p38 mitogen-activated protein kinase(p38 MAPK).Results:The SPT revealed that the sucrose preference rate of rats in the fatigue group was significantly lower than that of the control group(P＜0.01);Immunohistochemical staining showed that the expression level of SP in the hippocampal CA1 region of rats in the fatigue group was significantly increased compared with that of the control group(P＜0.01),and accom-panied by a significant increase in the activation level of MG in the hippocampal CA1(P＜0.05).In addition,West-ern Blot results indicated that exercise-induced fatigue led to a significant increase in the p-p38 MAPK/p38 MAPK val-ues in the hippocampus of rats(P＜0.01).Conclusion:The development of depressive-like behavior induced by exer-cise-induced fatigue is associated with upregulated SP secretion and excessive MG activation in the hippocampal CA1 region.The activation of the p38 MAPK pathway may be a regulatory mechanism underlying the adverse mood states caused by exercise-induced fatigue.
