The morphological feature of projections from the nucleus reuniens to the dorsal and ventral hippocampus
10.16557/j.cnki.1000-7547.2024.06.001
- VernacularTitle:小鼠丘脑连结核至背侧和腹侧海马通路的形态学特征
- Author:
Siting LYU
1
;
Yuankun LIU
;
Shumin WANG
;
Shuting REN
;
Sicong MA
;
Jing CHEN
;
Baoli LI
;
Juan SHI
Author Information
1. 延安大学医学院病原生物学教研室,延安 716000;空军军医大学基础医学院人体解剖与组织胚胎学教研室暨梁銶琚脑研究中心,西安 710032
- Publication Type:Journal Article
- Keywords:
nucleus reuniens(Re);
hippocampus;
recombinant adeno-associated virus(rAAV);
Fluoro-Gold(FG);
calretinin(CR);
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(6):663-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the neuroanatomical properties of the projection pathway from the nucleus reuniens(Re)to the dorsal(dHC)and ventral(vHC)hippocampus.Methods:Adeno-associated virus SV40 was injected into the Re of C57BL/6 mice and the profile of downstream projection in the whole brain was examined and ranked.Retro-grade tracer Fluoro-Gold(FG)was injected into the whole hippocampus(wHC),dorsal hippocampus(dHC)or ven-tral hippocampus(vHC),and the distribution of the retrogradely labeled neurons and its relationship with calretinin(CR)in the Re were analyzed.Results:After injection of SV40 virus into the Re,we observed projection fibers or ter-minals in 18 areas across the brain.Among the areas,those to the hippocampus(HC)were mainly distributed in the dorsal and ventral lacunosum molecular layer,which ranked No.2 with regard to the projection intensity.Following injection of FG into the hippocampus,we observed that the retrogradely labeled neurons projecting to the wHC were densely distributed in the rostrocaudal segments of Re,with a more concentrated aggregation in the ventromedial part.FG+CR+double labeled neurons accounted for(47.47±0.07)％of FG labeled neurons,and for(67.13±0.10)％of CR+neurons in this case.The retrograde labeled neurons projecting to the dHC were sparse and mainly distributed in the dorsolateral part of Re.FG+CR+double labeled neurons accounted for(24.11±0.06)％of FG labeled neurons,and for(32.99±0.19)％of CR+neurons.The retrogradely labeled neurons projecting to the vHC were mainly distrib-uted in the ventralmedial part of Re.FG+CR+double labeled neurons accounted for(49.55±0.03)％of FG labeled neurons,and for(69.14±0.12)％of CR+neurons.Conclusion:Hippocampus is an essential target of the Re.The projections to the dHC and vHP differ in the location of projection neurons and the positive ratio with CR,which may re-flect the differential pathophysiological functions of the two pathways in vivo.
