Neural mechanisms of psychological stress regulation on olfactory preferences
10.16557/j.cnki.1000-7547.2024.06.002
- VernacularTitle:压力应激调控小鼠气味偏好的神经机制研究
- Author:
Shiyu LUO
1
;
Qi HAN
1
Author Information
1. 上海交通大学医学院解剖学与生理学系,上海 200025
- Publication Type:Journal Article
- Keywords:
unpredictable mild stimulus(UMS);
odour preference test;
posterior dorsal part of medial amygdala(MePD);
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(6):673-680
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of psychological stress on odour preference choices of mice and the related neural mechanisms.Methods:C57BL/6 male mice were randomly divided into control group and stress group.An unpredictable mild stress(UMS)model was established in the stress group using mild stress stimuli like fasting,restraint,and circadian rhythm disruption combined with open field tests.Then,the preferences for food extract odour and female urine odour was examined in control and UMS male mice.The specific brain regions activated during dual odour preference tests in UMS mice were identified by the expression of the neuronal activity-related c-Fos.Further,chemogenetic manipulation was used to validate key brain areas influencing UMS mice's odour preferences.Results:Control mice showed no clear preference for either odour,while UMS mice demonstrated a significant preference for fe-male urine.During the dual odour preference test,an increase in c-Fos positive neurons is observed in the medial pre-optic area(MPOA)and posterior dorsal part of medial amygdala(MePD)of UMS mice.Activating MePD resulted in male mice preferring female urine odour,while inhibiting MePD reduced this preference.The manipulation of MPOA did not affect the outcome of the dual-choice odour preference test.Conclusion:UMS male mice has a significant prefer-ence for female urine odour,and MePD is a key brain region regulating this behavior.
