Aerobic exercise regulates autophagy to improve myocardial fibrosis in mice with atherosclerosis
10.3969/j.issn.1001-1242.2025.12.002
- VernacularTitle:有氧运动调控自噬改善动脉粥样硬化小鼠心肌纤维化机制研究
- Author:
Xi-hua LIU
1
;
Tiantian MA
;
Fang QIN
Author Information
1. 山东中医药大学附属医院,山东省 济南市,250014
- Publication Type:Journal Article
- Keywords:
aerobic exercise;
atherosclerosis;
myocardial tissue;
autophagy;
fibrosis
- From:
Chinese Journal of Rehabilitation Medicine
2025;40(12):1775-1781
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of aerobic exercise regulating autophagy to improve myocardial fibrosis in mice with atherosclerosis.Method:Eight weeks old male ApoE-/-knockout mice were randomly divided into model group,aerobic exer-cise group and blank control group after one-week adaption,with nine mice in each group.The atherosclerosis model was established by fed a"Western type"diet for 12 weeks and the aerobic exercise group was re-ceived aerobic training at the same time.After 12 weeks,Oil Red O staining was used to observe the patho-logical status of atherosclerosis in the two groups,and Masson dyeing was used to detect myocardial fibrosis in each group.The expressions and localization of ATG5,LC3BⅡ/Ⅰ,Beclin-1 and p62 in myocardial tissue of mice in each group were detected by immunofluorescence.The protein levels of ATG5,LC3BⅡ/Ⅰ,Beclin-1 and p62 in myocardial tissue of each group were detected by Western Blot.Result:Oil red O staining showed that obvious atherosclerotic plaque formation was found in the model group,while the area of atherosclerotic plaque in the aerobic exercise group was significantly less than that in the model group,indicating the degree of atherosclerosis was significantly improved.Masson dyeing showed that myocardial fibrosis in the aerobic exercise group was significantly improved compared with the model group,and the degree of myocardial fibrosis in both groups was greater than that in the blank control group.Immunofluorescence and Western Blot analysis showed that the expression levels of ATG5,LC3B II/I,and Be-clin-1 in the myocardial tissue of aerobic exercise group mice were significantly increased,while the level of p62 protein was significantly reduced(P<0.05).Conclusion:Aerobic exercise can significantly improve myocardial fibrosis in mice with atherosclerosis,and the mechanism may be related to aerobic exercise activated autophagy-related factors in myocardial tissue of mice.
