Advances in single-cell RNA sequencing in retinal development and disease initiation and progression
10.3760/cma.j.cn115989-20221028-00499
- VernacularTitle:单细胞转录组测序在视网膜发育及疾病发生和发展中的作用
- Author:
Manhong XU
1
;
Xiaorong LI
1
Author Information
1. 天津医科大学眼科医院 天津医科大学眼视光学院 天津医科大学眼科研究所 国家眼耳鼻喉疾病临床医学研究中心天津市分中心 天津市视网膜功能与疾病重点实验室，天津 300384
- Publication Type:Journal Article
- Keywords:
Single-cell RNA sequencing;
Retina;
Retinal diseases;
Genetic markers;
Cellular heterogeneity
- From:
Chinese Journal of Experimental Ophthalmology
2025;43(6):571-576
- CountryChina
- Language:Chinese
-
Abstract:
Single-cell RNA sequencing (scRNA-seq) is an unbiased, high-throughput, and high-resolution transcriptome analysis method at the single-cell level.Unlike ordinary transcriptome sequencing, which study the average gene expression of all cells in tissues, scRNA-seq maps RNA to individual cells, which can be used to discover new cell types, reveal cell heterogeneity, and identify rare cells.Recently, more and more researches on scRNA-seq have focused on ophthalmology.This technology is expected to reveal the complex developmental biological processes of the retina and the pathogenesis of diseases, providing guidance for the clinical treatment of ophthalmology.This article briefly introduces the development and technical process of scRNA-seq, describes the identification of various retinal cell subtypes, the retinal cell development, and the application of retinal organoids in detail.This article systematically summarizes the latest research progress on scRNA-seq in the pathogenesis, diagnosis and treatment of retinal diseases, such as diabetic retinopathy and age-related macular degeneration, which mainly focuses on changes in cell-specific molecules and regulatory pathways in the occurrence and development of diseases, which will be helpful to search for potential new treatment targets for retinal diseases.
