Effect of Graft Composition on Viral Infection and Survival after Allogeneic Hematopoietic Stem Cell Transplantation
10.19746/j.cnki.issn1009-2137.2025.06.039
- VernacularTitle:移植物成分对异基因造血干细胞移植后病毒感染及生存的影响
- Author:
Fan LIU
1
;
Shan WANG
1
;
Qiu-Juan ZHU
1
;
Tao WANG
1
;
Wei-Wei TIAN
1
;
Rong GONG
1
;
Zhi-Lin GAO
1
Author Information
1. 山西医科大学第三医院(山西白求恩医院/山西医学科学院/同济山西医院)血液科,山西太原 030032
- Publication Type:Journal Article
- Keywords:
allogeneic hematopoietic stem cell transplantation;
graft;
mononuclear cells;
viral infection
- From:
Journal of Experimental Hematology
2025;33(6):1803-1808
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of infusing different doses of graft cells on viral infections and survival after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods:Clinical data of 99 patients with hematological diseases who underwent allo-HSCT in Shanxi Bethune Hospital were retrospectively analysed.The proportion of mononuclear cells(MNC)was counted microscopically,the proportions and absolute counts of CD34+cells and lymphocyte subsets(including CD3+T,CD4+T,CD8+T,CD4+CD8+T,B,and NK cells)were detected by flow cytometry,and the infusion dose of each cell types was calculated.The patients were divided into high-dose group and low-dose group based on the median value of the infused cell dose.The effects of different doses of various graft cells on post-transplant viral infections(CMV,EBV,BKV)and survival were analyzed.Results:The low-dose MNC(＜7.97 × 108/kg)and high-dose CD4+CD8+T cell(≥3.02 × 106/kg)groups had a higher Epstein-Barr virus(EBV)infection rate(P=0.031;P=0.020).The high-dose CD34+cell group and low-dose CD3+T,CD8+T and natural killer(NK)cell groups showed a trend toward higher EBV infection rates,but the differences were not statitsically significant(P＞0.05).There was no significant difference in the rates of EBV infection between the high-dose and low-dose groups of CD4+T and B cells(P＞0.05).Multivariate analysis revealed that the doses of MNC and CD4+CD8+T cells in the graft were independent factors influencing EBV infection(P=0.023;P=0.016).The doses of cells in the graft showed no significant impact on CMV and BKV infection,or patient survival(P＞0.05).Conclusion:Infusing a lower dose of MNC and a higher dose of CD4+CD8+T cells increases the risk of EBV infection,but does not affect the survival outcome.
