Role of the extracellular signal-regulated kinase pathway in arsenic-induced apoptosis in mouse hippocampal neuron cells
10.12007/j.issn.0258-4646.2025.01.005
- VernacularTitle:胞外信号调节激酶信号通路对亚砷酸钠诱导的小鼠海马神经元细胞凋亡的调控作用
- Author:
Yao CHEN
1
;
Man YU
;
Xin LI
;
Yuting WEI
;
Xudan LIU
;
Huanhuan WANG
;
Mei WANG
Author Information
1. 辽宁中医药大学中西医结合学院公共卫生与预防医学教研室,沈阳 110847
- Publication Type:Journal Article
- Keywords:
arsenic;
extracellular signal-regulated kinase;
cAMP response element binding protein;
apoptosis;
nerve injury
- From:
Journal of China Medical University
2025;54(1):24-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of reactive oxygen species(ROS)and the extracellular signal-regulated kinase(ERK)/cAMP response element binding protein(CREB)signaling pathway in arsenic-induced apoptosis in HT-22 cells.Methods HT-22 cells were cultured in vitro and exposed to NaAsO2.The cells were divided into the following groups:control group,4,6,8,and 10 μmol/L NaAsO2 groups,NAC(5 mmol/L)group,and NAC(5 mmol/L)+NaAsO2(10 μmol/L)group.ROS levels were measured using a DCFH-DA assay.The expression of ERK/CREB signaling pathway-related and apoptosis-related proteins were analyzed by Western blotting.Apop-tosis rates were evaluated using flow cytometry.Results Compared with the control group,ROS levels in HT-22 cells significantly increased,while p-ERK protein level in the nucleus and p-CREB and Bcl-2 protein levels in the cells significantly decreased in the NaAsO2 only exposed groups(P＜0.05).Additionally,the apoptosis rate significantly increased in the 8 and 10 μmol/L NaAsO2 groups compared with the control group(P＜0.05).In the NAC+NaAsO2 group,ROS levels and the apoptosis rate significantly decreased while p-ERK protein level in the nucleus and p-CREB and Bcl-2 protein levels in the cells significantly increased compared with the 10 μmol/L NaAsO2 group(P＜0.05).Conclusion Arsenic exposure induces oxidative stress,inhibits the nuclear translocation of p-ERK,and dis-rupts the CREB signaling pathway,leading to apoptosis.
