Effects and mechanism on icariin for osteoporosis in diabetic rats
10.12007/j.issn.0258-4646.2025.01.003
- VernacularTitle:淫羊藿苷对糖尿病大鼠骨质疏松症的作用及其机制
- Author:
Chao MAN
1
;
Yansong WANG
Author Information
1. 锦州医科大学第一临床学院,辽宁锦州 121000
- Publication Type:Journal Article
- Keywords:
icariin;
diabetic osteoporosis;
estrogen receptor 1;
Wnt/β-catenin signaling pathway
- From:
Journal of China Medical University
2025;54(1):12-17,23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of icariin on diabetic osteoporosis(DOP)in rats by regulating estrogen reoeptor 1(ER1).Methods Fifty SD rats were randomly divided into five groups:control,diabetes(STZ),low-dose icariin[four weeks after successful establishment of diabetic model rats,50 mg/(kg·d)icariin was administered by gavage for eight weeks],high-dose icariin[100 mg/(kg·d)icariin administered by gavage for eight weeks],and high-dose icariin+ER1-siRNA group[icariin 100 mg/(kg·d)administered by gavage for eight weeks.A total of 10 μL ER1-siRNA was injected via the tail vein once every four weeks,two times in total],with 10 rats in each group.HE staining was used to detect the pathological morphology of the femoral tissue.Enzyme-linked immunosorbent assay(ELISA)was used to measure serum ALP,TRAP,and blood calcium concentrations.Protein-protein interaction(PPI)network analysis predicted icariin core targets and validated molecular docking.ER1,PDE5A,Wnt,and β-cantenin expression levels in rat femoral tissue were detected using Western blotting.Results In the STZ group,the number of empty lacunae in the subchondral region increased,ALP expression and blood calcium concentration decreased(P＜0.01),and TRAP expression increased(P＜0.01),compared with the control group.In the low-and high-dose icariin groups,blood glucose levels decreased(P＜0.01),the number of empty bone lacunae in the subchondral region decreased,ALP expression and blood calcium concentration increased(P＜0.01),and TRAP expression decreased(P＜0.01),compared with the STZ group.PPI network analysis and molecular docking showed that icariin stably bound to the ER1 protein structure.Com-pared with the control group,expression of ER1,Wnt,and β-cantenin decreased in STZ group(P＜0.01).The expression of ER1,Wnt,and β-cantenin in the high-dose icariin group increased compared with the STZ group(P＜0.01).Moreover,expression of ER 1,Wnt,andβ-cantenin decreased in the high-dose icariin+ER1-siRNA group,compared with the high-dose icariin group(P＜0.01).The protective effects of icariin were reversed by ER1-siRNA administration.Conclusion Icariin could significantly improve osteoporosis in diabetes rats,which may be relevant to the activation of the ER 1-mediated Wnt/β-cantenin pathway.
