Effect of Shenghua Sanjia decoction on endometrial damage after induced abortion in rats by regulating Fas/FasL signaling pathway
10.12007/j.issn.0258-4646.2025.01.006
- VernacularTitle:生化散瘕汤调控Fas/FasL信号通路对人工流产后大鼠子宫内膜损伤的影响
- Author:
Jiuyan ZHANG
1
;
Juanjuan SHI
;
Dan ZHANG
Author Information
1. 滕州市中心人民医院药学部,山东滕州 277599
- Publication Type:Journal Article
- Keywords:
Shenghua Sanjia decoction;
induced abortion;
Fas/Fas ligand signaling pathway;
endometrial damage;
rat
- From:
Journal of China Medical University
2025;54(1):30-37,43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the Shenghua Sanjia decoction on endometrial damage after induced abortion in rats by regulating the Fas/Fas ligand(FasL)signaling pathway.Methods Fifty pregnant rats were randomly assigned to five groups:normal con-trol,model,low-and high-dose Shenghua Sanjia decoction(12.6 g/kg and 25.2 g/kg,respectively),and dydrogesterone(3 mg/kg)groups,with 10 rats in each group.In all groups except the normal control group,rats were administered 16.6 mg/kg mifepristone combined with 100 μg/kg misoprostol to establish an artificial abortion model,and the endometrial injury model was established by a mechanical injury method.After administering the respective drug intervention,enzyme-linked immunosorbent assay(ELISA)was used to measure serum levels of estrogen(E2),progesterone(P),and prolactin(PRL).Histopathological changes of the endometrium were evaluated using HE staining.Masson staining was employed to assess endometrial fibrosis,whereas TdT-mediated dUTP nick-end labeling(TUNEL)was uti-lized to detect apoptosis in the endometrial tissue.Additionally,the mRNA expression levels of the estrogen receptor(ER),progesterone receptor(PR),prolactin receptor(PR LR),vascular endothelial growth factor(VEGF),Fas,and FasL in the endometrial tissue were measu-red using real-time quantitative PCR.Finally,Western blotting was performed to analyze the protein expression levels of transforming growth factor-β(TGF-β),matrix metalloproteinase-9(MMP-9),Fas,and FasL in the endometrial tissue.Results Compared to the normal control group,the model group showed a decreased E2,P,and PRL serum levels,a reduction in the mRNA expressions of ER,PR,PRLR,and VEGF as well as decreased protein expression of MMP-9 in the endometrial tissue.However,the apoptosis rate,mRNA expression of Fas and FasL,and protein expression of TGF-β,Fas,and FasL in the endometrial tissue were all increased(P＜0.05).Lastly,the endo-metrial tissue was found to be severely damaged with the collagen fibers increased.Compared to the model group,the low-and high-dose Shenghua Sanjia decoction and dydrogesterone groups showed an increase in the serum levels of E2,P,and PRL,as well as an elevation in the mRNA expression of ER,PR,PRLR,and VEGF;similarly the protein expression of MMP-9 in the endometrial tissue was increased.However,the apoptosis rate,mRNA expression of Fas and FasL,and protein expression of TGF-β,Fas,and FasL in the endometrial tissue were all decreased(P＜0.05).Conversely,the histopathological damage of the endometrium was found to be alleviated with the collagen fibers decreased.Conclusion The Shenghua Sanjia decoction can reduce endometrial fibrosis and apoptosis,while promoting endome-trial repair after the induction of abortion in rats.The underlying mechanism may be associated with the inhibition of the Fas/FasL signa-ling pathway activity.
