The Mechanism of Echinococcus Granulosus Sensu Stricto Antigen B to Protect Immune Thrombocytopenia Mouse Model by Influen-cing Autophagy
10.19746/j.cnki.issn1009-2137.2025.06.021
- VernacularTitle:细粒棘球绦虫抗原B通过影响自噬以保护免疫性血小板减少症模型小鼠的作用机制
- Author:
Hai-Chen SONG
1
;
Xue-Mei WANG
1
;
Dan-Lu LI
1
;
Li ZHAO
1
;
Xue-Hua YANG
1
;
Mei YAN
1
Author Information
1. 新疆医科大学第一附属医院儿内一科,新疆乌鲁木齐 830054
- Publication Type:Journal Article
- Keywords:
immune thrombocytopenia;
Echinococcus granulosus sensu stricto antigen B;
autophagy;
immunoregulation
- From:
Journal of Experimental Hematology
2025;33(6):1694-1700
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of natural antigen B(nAgB)to protect Immune thrombocytopenia(ITP)mouse model by influencing autophagy.Methods:Twenty-eight female BALB/c mice aged 8-10 weeks were randomly divided into four groups.7 mice of each group were immunized intraperitoneally,the control group was treated with PBS as the control group;ITP group was treated with anti-CD41 monoclonal antibody(anti-CD41Ab)only;nAgB group was treated with nAgB intraperitoneal injection for 5d;nAgB+ITP group was treated with nAgB intraperitoneal injection for 5d,then treated with anti-CD41 Ab.The peripheral blood platelet count in each group was tested;and the spleen and liver should be isolated and weighed,the organ index was calculated;qRT-PCR was used to detect spleen microtubule-associated protein 1 light chain 3(LC3),p62,Beclin-1 mRNA expression levels.Western blot was used to detect the protein expression level of spleen LC3 Ⅱ/LC3 Ⅰ,p62,Beclin-1.Results:Compared with the control group,mice in the ITP group showed a significant decrease in blood PLT count[(102.1±17.9)× 109/L vs(485.4±185.2)×109/L,P＜0.01],a significant increase in spleen index(P＜0.01),mice in the nAgB group showed a significant increase in blood PLT count,rising to(1051±127.6)× 109/L on the 3 day after modeling.Compared with the ITP group,mice in the nAgB+ITP group showed a significant increase in PLT count on the 1 day of anti-CD41 Ab administration[(428.6±131.6)× 109/L vs(102.1±17.9)×109/L,P＜0.05],however,the spleen index was significantly decreased(P＜0.05).qRT-PCR and Western blot results showed that compared with the control group,the mRNA and protein expression levels of spleen LC3,p62 and Beclin-1 were increased in the ITP group of mice(P＜0.05,P＜0.01).Compared with the ITP group,the nAgB+ITP group could significantly decrease mRNA levels of spleen LC3,p62 and Beclin-1(P＜0.05,P＜0.01),and also significantly decrease the protein expression levels of LC3 Ⅱ/LC3 Ⅰ,p62 and Beclin-1(P＜0.05,P＜0.01).Conclusion:nAgB inhibits the transcription and expression levels of autophagy-related genes and regulates immune intolerance,thereby protecting ITP mouse models.
