Detection research of diatom by gene arrays in drowning cases
10.13618/j.issn.1001-5728.2025.01.010
- VernacularTitle:硅藻基因芯片在溺亡检验中的应用研究
- Author:
Ping YANG
1
;
Yongjun XIA
;
Min RAO
;
Zhihao ZHANG
;
Bo XIE
;
Weiwen CAI
;
Xiaoli WANG
Author Information
1. 中山市公安局刑事科学技术研究所,广东 中山 528400
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Diatoms detection;
Gene arrays;
Drowning;
Evaluation of application
- From:
Chinese Journal of Forensic Medicine
2025;40(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect diatoms in tissues and water samples from drowning cases by gene array,and explore the application value of DNA microarray.Methods Diatoms in the lung,liver,kidney samples,and on-site water samples from 19 drowned corpses that appeared in the rivers of Zhongshan City between July 2021 and April 2022,were detected by gene arrays.Moreover,diatom detection was also performed on 28 samples from 7 corpses by microscope.Then the testing results were compared and analyzed.Results For those 28 samples,diatom types in both tissue and water samples by gene detection were more than those detected by microscopy,the detection was statistically significant(P<0.05).However,there was no statistically significant difference(P>0.05)in the detection of diatom types between tissue samples and water samples both using gene arrays.In terms of tissue samples,diatoms were easier to be detected in lung than liver or kidney,and there were also more types of diatoms detected in lung.Diatom detection by gene arrays showed that the diatom types showed a decreasing trend as the tissue was further away from the entrance of body,and the difference of diatom types increased among tissues.Conclusion The gene array can effectively detect the diatom types and distribution characteristics in drowned corpses,and has great potential in the research of the traceability analysis of corpses found in water and the causes of death.
