Biomarkers affecting the progression of mild to moderate cognitive impairment after stroke:a non-targeted metabolomics analysis
- VernacularTitle:非靶向代谢组学分析影响轻中度脑卒中后认知功能障碍的生物标记物
- Author:
Zhifeng WANG
1
;
Jiao YANG
;
Yujiang XI
;
Shuangfeng XU
;
Ting SHI
;
Junfeng LAN
;
Zhihui HAO
;
Pengfen HE
;
Aiming YANG
;
Pan PAN
;
Jian WANG
Author Information
- Publication Type:Journal Article
- Keywords: post-stroke cognitive impairment; non-targeted metabolomics; ultra performance liquid chromatography-mass spectrometry; biomarker; differential metabolites; area under the curve; variable importance in projection
- From: Chinese Journal of Tissue Engineering Research 2025;29(24):5116-5126
- CountryChina
- Language:Chinese
- Abstract: BACKGROUND:Cognitive impairment is the most common complication after stroke,and its severity is closely related to the patient's prognosis.The prognosis of patients can be significantly improved if the severity of their cognitive impairment is recognized and targeted early.OBJECTIVE:To initially explore potential biomarkers affecting the progression of post-stroke cognitive impairment,thereby providing a richer and unique reference for the study of their pathophysiological mechanisms.METHODS:Using ultra performance liquid chromatography-mass spectrometry,non-targeted metabolomics analysis was conducted on serum samples from patients with mild and moderate post-stroke cognitive impairment to identify differential metabolites between the two groups.To further validate the diagnostic efficacy of the differential metabolites,the receiver operating characteristic curve analysis was used to evaluate their accuracy and sensitivity in distinguishing disease severity.In addition,pathway analysis was conducted on the differential metabolites.RESULTS AND CONCLUSION:(1)There were significant differences in metabolic profiles between patients with mild and moderate post-stroke cognitive impairment,and 9 differential metabolites were screened by the receiver operating characteristic curve.(2)Differential metabolite pathway analysis revealed that the metabolic pathways affecting disease progression in patients with mild-to-moderate post-stroke cognitive impairment included tryptophan metabolism,D-amino acid metabolism,biotin metabolism,retinol metabolism,aminoacyl-tRNA biosynthesis,lysine degradation,protein digestion and uptake,pyrimidine metabolism,cysteine and methionine metabolism,ABC transporter proteins,amino acid biosynthesis,and 2-oxocarboxylic acid metabolism.To conclude,9 potential biomarkers affecting disease progression in patients with mild-to-moderate post-stroke cognitive impairment have been identified,involving 12 metabolic pathways including tryptophan metabolism,D-amino acid metabolism and retinol metabolism.
