Similarity Between Young Fruits of Citrus aurantium'Changshan-huyou'and Aurantii Fructus Immaturus Based on HPLC Fingerprint and Chemometrics
10.3870/j.issn.1004-0781.2025.02.016
- VernacularTitle:基于HPLC指纹图谱与化学计量学研究常山柚橙幼果与枳实的相似性
- Author:
Xiaoxiao WANG
1
;
Weinan HU
;
Jian WANG
;
Jianli ZHENG
;
Xiaofeng SHAO
;
Fei LUO
Author Information
1. 衢州市食品药品检验研究院,衢州 324002
- Publication Type:Journal Article
- Keywords:
Citrus aurantium'Changshan-huyou';
Aurantii Fructus Immaturus;
Fingerprint;
High performance liquid chromatography;
Chemometrics
- From:
Herald of Medicine
2025;44(2):273-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the similarity between young fruits of Citrus aurantium'Changshan-huyou'and Pharmacopoeia origin Aurantii Fructus Immaturus.Methods The fingerprints of young fruits of Citrus aurantium'Changshan-huyou'and the Pharmacopoeia origin Aurantii Fructus Immaturus were established by high performance liquid chromatography(HPLC).The similarity analysis was carried out by using the software of Traditional Chinese medicine chromatographic fingerprint similarity evaluation system.The whole components of young fruit of Citrus aurantium'Changshan-huyou'and the Pharmacopoeia origin Aurantii Fructus Immaturus were compared by using chemometrics,and the 14 compounds were analyzed quantitatively.Results ①The results of similarity and cluster analysis were affected by the origin and producing area,for example,the similarity between citrus sinensis(L.)Osbeck and other origin was only 0.2,the similarity of citrus aurantium L.from different producing areas ranged from 0.802 to 0.986,with relative large dispersion;②young fruit of Citrus aurantium'Changshan-huyou',some citrus aurantium L.,citrus aurantium L.cv.Daidai,stink orange and citrus aurantium L.cv.Zhulan all belong to the same category,and the similarity was above 0.9;③VIP method screened out 21 common peaks that had important effects on quality,mainly including neohesperidin,naringin,umbellitin,tangeretin,hesperidin hydrate and hesperidin.The quantitative analysis results showed that Naringin and neohesperidin were abundant in young fruits of Citrus aurantium'Changshan-huyou'and Aurantii Fructus Immaturus,followed by hesperidin and naringin,there were significant differences in the content of principal components among Aurantii Fructus Immaturus from different origins,especially in citrus sinensis(L.)Osbeck,the young fruits of Citrus aurantium'Changshan-huyou'was relatively stable.Conclusion The young fruits of Citrus aurantium'Changshan-huyou'had good similarity with most origin of Aurantii Fructus Immaturus,so it is feasible to be used as Aurantii Fructus Immaturus.
