Effects of Chrysin on the Intestinal Flora in Mice with Alcoholic Liver Disease Model
10.3870/j.issn.1004-0781.2025.02.002
- VernacularTitle:白杨素对酒精性肝病模型小鼠肠道菌群的影响
- Author:
Lu DONG
1
;
Haotian ZHANG
1
;
Yanyu KANG
1
;
Fei WANG
1
;
Haolin GUO
1
;
Ying DONG
1
;
Yong YANG
1
;
Ting BAI
1
Author Information
1. 大连大学慢性病研究中心大连市重点实验室,大连 116622
- Publication Type:Journal Article
- Keywords:
Chrysin;
Alcoholic liver disease;
Intestinal flora;
16s rDNA sequencing;
g-Firmicutes;
Alloprevotella
- From:
Herald of Medicine
2025;44(2):176-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of chrysin on intestinal flora in mice with alcoholic liver disease(ALD).Methods Mice were randomly assigned to normal control group,ALD model group,Silymarin group,chrysin low-dose group,chrysin high-dose group(25,50 mg·kg-1).The mice were fed with alcoholic liquid diet and a single dose of alcohol(5 g·kg-1)for eight weeks to establish the ALD model.After eight weeks of oral administration,each group's serum and plasma lipids and liver function indices were collected and detected using kits;then collected the liver and observed the pathological changes of the liver using HE staining;meanwhile,intestinal contents were collected and changes in mouse gut flora were analyzed by 16S rDNA sequencing.Results Compared with the ALD group,the level of aspartate transaminase(AST),alanine transaminase(ALT)and triacylglycerol(TG)of low-dose and high-dose chrysin groups were significantly reduced,and it can alleviate liver cell steatosis and inflammatory reactions caused by alcohol.16S rDNA results showed that the total number and types of intestinal flora in the ethanol group were significantly reduced,as well as a change in the dominant genus to Escherichia-Shigella and Akkermansia.Compared to the ALD model group,the Shannon index of the intestinal microbiota increased significantly in mice treated with low and high doses of chrysin.In addition,at the phylum and genus level,the abundance of the high-dose chrysin group increased significantly,resulting in an overall increase in the total number and amount of microbiota.The abundance of dominant bacterial groups,such as Oscillospirales,irmicutes,andAlloprevotella,was also significantly increased.Conclusion Chrysin may exert therapeutic effects on ALD by improving intestinal flora imbalance in ALD mice.
