Detection of cerebral ischemia-reperfusion injury using BIBP-H fluorescent probe
10.3969/j.issn.1006-7795.2025.01.013
- VernacularTitle:利用BIBP-H荧光探针进行脑缺血再灌注损伤检测
- Author:
Wei CUI
1
;
Qidan PANG
;
Hanyue XIANG
;
Nao XIAO
;
Dechun JIANG
;
Shen LI
;
Guangli SHEN
Author Information
1. 首都医科大学附属北京世纪坛医院神经与精神科,北京 100038
- Publication Type:Journal Article
- Keywords:
fluorescent probe;
cerebral ischemia/reperfusion;
oxidative stress;
biological imaging
- From:
Journal of Capital Medical University
2025;46(1):76-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the potential of the BIBP-H fluorescent probe in the detection of the oxidative stress levels after cerebral ischemia-reperfusion(CIRI).Methods In vitro,the potential of BIBP-H probe was in detection of oxidative stress was first assessed with fluorescence imaging in rat neuroblastoma(B104)cells after L-glutamic acid stimulation.And then,the effects of edaravone and dexborneol(EDA)and glutathione(GSH)pretreatment on the fluorescence intensity were evaluated.Later,a totally of 28 male C57BL/6 mice were randomly assigned into four groups:transient middle cerebral artery occlusion(tMCAO)group,EDA+tMCAO group,GSH+tMCAO group,and sham group.After 1.5 h ischemia and 12 h reperfusion,the mice were treated with BIBP-H via tail vein injection.In vivo,ex vivo,and tissue fluorescence imaging were utilized to evaluate the probe's cerebral ischemia-reperfusion injury(CIRI).Results ① BIBP-H probe did not exhibit fluorescence signals in cultured B104 cells,but showed red fluorescence in B104 cells treated with L-glutamic acid.The signals significantly decreased when pretreated with EDA or GSH.② Both the sham-operated mice intravenously injected with the BIBP-H probe and the tMCAO mice without injection of the probe showed negative results in in vivo fluorescence imaging.③ tMCAO mice treated with BIBP-H exhibited red fluorescence signals in the ischemic hemisphere in vivo,with significantly reduced fluorescence intensity after EDA or GSH infusion during reperfusion ④ The fluorescence area examined with BIBP-H was consistent the cerebral infarction area detected with triphenyltertrazolium.Conclusions The BIBP-H probe effectively monitored oxidative stress levels both in vivo and in vitro,demonstrating its potential in CIRI detection.
