Clinical application evaluation of magnetic particle chemiluminescence immunoassay for determination of fungus(1,3)-β-D glucan in serum
10.13602/j.cnki.jcls.2025.11.11
- VernacularTitle:磁微粒化学发光免疫试验检测血清中真菌(1,3)-β-D葡聚糖的临床应用评价
- Author:
Ying WANG
1
;
Hongwei PAN
1
;
Wei LI
1
;
Enhua SUN
1
Author Information
1. 山东大学齐鲁医院检验科,济南 250014
- Publication Type:Journal Article
- Keywords:
(1,3)-β-D glucan;
invasive fungal disease;
magnetic particle chemiluminescence immunoassay
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(11):857-860
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the clinical value of fungus(1,3)-β-D glucan test magnetic particle chemiluminescence immunoas-say(G-CLIA)for diagnosis of invasive fungal disease(IFD).Methods A total of 509 patients with clinically suspected IFD in Qilu Hospital of Shandong University from 1 March to 30 April,2023 were collected.According to the inclusion criteria,the 509 patients were grouped into IFD group(141 patients)and non-IFD group(368 patients).The sensitivity,specificity,accuracy,positive predic-tive value and negative predictive value of G-CLIA were analyzed,and the consistency of the results of G-CLIA with G test colorimetric method,fungal smear microscopy or culture and metagenomics next-generation sequencing(mNGS)was comparatively analyzed.Re-sults The sensitivity and specificity of G-CLIA were 88.65％and 96.47％,respectively,and the positive percentage agreement of G-CLIA with G test colorimetric assay,fungal smear microscopy or culture,and mNGS were 92.19％,75.86％,and 75.00％,respective-ly,and the consistency of G-CLIA with G test colorimetric assay was the highest(kappa value≥ 0.75).Conclusion G-CLIA has high sensitivity and specificity for detecting IFD with excellent diagnostic value.Combined with the fully automated chemiluminescence analy-zer,G-CLIA test is fast and has a high throughput,which provides a new option for the clinical diagnosis of IFD.
