High-throughput Sequencing Analysis of Reproductive Tract Microbiota in Pa-tients with Moderate and Severe Intrauterine Adhesions
- VernacularTitle:中重度宫腔粘连患者生殖道微生态高通量测序分析
- Author:
Mingqian CHEN
1
;
Bao LIU
1
;
Yugang CHI
1
Author Information
1. 重庆市妇幼保健院 重庆医科大学附属妇女儿童医院妇产科,重庆 401120
- Publication Type:Journal Article
- Keywords:
Intrauterine adhesion;
Reproductive tract microbiota;
Endometrial microbiota;
High-throughput se-quencing
- From:
Journal of Practical Obstetrics and Gynecology
2025;41(1):77-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the differences in reproductive tract microbiota between patients with moder-ate and severe intrauterine adhesions(IUA)and infertile patients with normal uterine cavities and the clinical sig-nificance.Methods:Thirty patients were enrolled in the study from June 1,2022 to September 12,2022.Among them,15 cases were confirmed as moderate to severe IUA by hysteroscopy(group A),and 15 cases of primary infertility were excluded from intrauterine diseases through outpatient hysteroscopy(group B).Perform high-throughput sequencing of secretions from the vagina,cervix,and uterine cavity separately.Results:There is no difference in the α diversity of intrauterine microbiota between the two groups,but the α diversity of intrauterine mi-crobiota in group A is significantly higher than that of cervical microbiota(P=0.036).At the genus level,the top 10 relative abundances are the same for both groups.At the species level,the abundance of lactobacillus iners in group A is significantly lower than that in group B(P=0.001).There is no statistically significant difference in the abundance of microorganisms in the vagina,cervix and uterine cavity of group A.Conclusions:The types of en-dometrial microbiota in IUA patients and infertile women are roughly the same,but the composition is different.There are differences in the diversity of reproductive tract microbiota in patients with IUA,with a higher diversity of microbiota in the uterine cavity.Which suggests that the pathogenesis of IUA may be related to the changes of endometrial microbiota,increased microbial diversity,complex microbial species,especially the imbalance of the microecology of lactobacillus,which is not conducive to the repair of endometrial damage.
