Differentially-expressed circRNA_06886 and its target genes in prefrontal cortex of schizophrenia mice
10.3760/cma.j.cn371468-20241209-00584
- VernacularTitle:circRNA-06886在精神分裂症模型小鼠前额皮质中的异常表达及其靶基因分析
- Author:
Xiaoli ZHU
1
;
Shengdong CHEN
;
Wei WANG
;
Lingming KONG
;
Haoyue WANG
;
Lei ZHOU
Author Information
1. 联勤保障部队第九〇四医院常州医疗区心理中心，常州 213000
- Publication Type:Journal Article
- Keywords:
Schizophrenia;
circRNA;
Olanzapine;
Dopamine pathway;
Bioinformatics;
Mice
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2025;34(9):783-789
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of olanzapine (OLZ) on the differentially-expressed circRNAs in prefrontal cortex of schizophrenia mouse models and predict the target genes.Methods:SPF grade C57BL/6 mice, 7~8 weeks-old, 20 male mice and 45 female mice were recruited and breeded offspring.Forty-four double-stimulation induced schizophrenia-like mouse models, the offspring mice exposed to dual stress were divided into the schizophrenia group(SZ group, n=23) and the olanzapine intervention group (SZ+ OLZ group, n=21), while the mice raised under normal conditions served as the control group (NC group, n=22). Whole transcriptome sequencing was used to sequence the expression level of RNAs from the prefrontal cortex of the mice. RT-qPCR was applied to verify the differentially-expressed circRNAs, then the target genes of miRNAs which have binding site to verified circRNAs were predicted. Results:RNA-seq results showed that there were 137 differentially-expressed circRNAs compared with NC group, 62 were significantly high-expressed and 75 were low-expressed. circRNA_06886 showed significant low-expressed in SZ group compared with NC group( Z=-3.259, P<0.01), and significant high-expressed in SZ+ OLZ group compared with SZ group( Z=-4.765, P<0.01). Bioinformatics analysis of miRNA target genes showed that the target genes were involved in the pathways related to neural pathways such as dopamine, glutamate and MAPK signaling pathways. Conclusions:There are differentially expressed circRNAs in the prefrontal cortex of schizophrenia mouse models, and circRNA_06886 is low-expressed in the prefrontal cortex of schizophrenia mice, Camk2b-201 and Plcb1-003 are the potential genes of circRNA_06886 involved in the regulation of schizophrenia pathogenesis by dopamine pathway.
