Screening of lncRNAs Associated with Muscle Development and Meat Formation in the Longest Dorsal Muscles of Female Yanchi Tan Sheep of Different Months of Age by Transcriptome Sequencing
10.13865/j.cnki.cjbmb.2024.12.1268
- VernacularTitle:盐池滩羊背最长肌中与肌肉发育和肉质形成相关lncRNA的筛选与功能分析
- Author:
Yi-Lan LIU
1
;
Ya-Mei HU
;
Hui-Xia LI
;
Jia-Wei LU
;
Yuan LIU
Author Information
1. 南京农业大学动物科技学院动物繁殖科学系草食动物教研室,南京 210000
- Publication Type:Journal Article
- Keywords:
Tan sheep;
muscle development;
meat quality;
long non-coding RNA(lncRNAs);
tran-scriptome sequencing(RNA-seq)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(2):284-295
- CountryChina
- Language:Chinese
-
Abstract:
Yanchi Tan sheep,a specialty of Ningxia,China,is famous for its excellent meat quality,but the detailed mechanisms of its muscle development and meat quality formation are still unclear.In or-der to preliminarily investigate the unique molecular mechanisms in the muscle development of the sheep,the present study was carried out to collect the longest dorsal muscle(LDM)samples from 6-month-old and 12-month-old female Tan sheep,and we screened the genes and long non-coding RNA(lncRNAs)that may play important roles in regulating the muscle development and meat quality formation through transcriptome sequencing(RNA-seq).The results showed that a total of 504 mRNAs and 1,483 lncRNAs were detected between the two groups of LDMs,with significant differences(P＜0.05).And we also pre-dicted 103 potential lncRNA-mRNA cis-regulatory relationship pairs with significant differences(P＜0.05).Skeletal muscle development-related genes such as MSTN,CDKN1A,FHL1 and skeletal muscle metabolism-related genes such as LPL and SCD were subsequently screened based on the functional anal-ysis of differentially expressed mRNA.Based on the functional analysis of differentially expressed lncRNA target genes,13 lncRNAs potentially related to muscle development(LNC.17926.1,LNC.981.1,LNC.16284.1,etc.)and 8 lncRNAs potentially related to skeletal muscle metabolism(LNC.15496.1,LNC.18149.1,etc.)were identified.5 lncRNAs and 4 mRNAs expressed in both groups were randomly selected for qPCR validation experiments,and their results were consistent with RNA-seq,which corrob-orated with the reliability of the sequencing results.This study lays a solid foundation for future investiga-tions of muscle development,meat quality formation mechanism and molecular breeding in Tan sheep,and provides a scientific basis for improving the meat production performance of other domestic sheep breeds.
