Pan-cancer Analysis of Long Chain Non-coding RNA KCNQ1OT1 and Its Regulatory Role on Glutamine Metabolism in Gastric Cancer
10.13865/j.cnki.cjbmb.2024.12.1242
- VernacularTitle:长非编码RNA KCNQ1OT1的泛癌分析及其在胃癌中对谷氨酰胺代谢的调控作用
- Author:
Ya-Nan YU
1
;
Jia-Qiu LI
1
;
Xiao-Lin MA
1
Author Information
1. 潍坊医学院附属医院肿瘤科,山东潍坊 261031
- Publication Type:Journal Article
- Keywords:
KCNQ1OT1;
pan-cancer;
biomarker;
glutamine metabolism;
enhancer
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(1):156-168
- CountryChina
- Language:Chinese
-
Abstract:
The long non-coding RNA KCNQ1OT1 plays an important role in promoting the occurrence and development of various cancers.However,there is currently no systematic analysis of KCNQ1OT1 in pan cancer.To elucidate the value of KCNQ1OT1 in tumor diagnosis and prognosis,this study analyzed its expression levels in pan-cancer tissues and its impact on patient prognosis.By analyzing the regulatory mechanism of KCNQ1OT1 in gastric cancer,new molecular targets may be found for the diagnosis and treatment of gastric cancer.Using Sangerbox 3.0,ACLBI and UALCAN databases,we found the expres-sion levels of KCNQ1OT1 were increased in 7 tumor tissues types(P＜0.05).We found KCNQ1OT1 ex-pression was correlated with poor prognosis in many tumor types using Sangerbox 3.0 database.We used R software to analyze the differential genes between the high and low expression groups of KCNQ1OT1 in gastric cancer patients(P＜0.05,|log2FoldChange|＞1).The GO and KEGG enrichment analysis showed that KCNQ1OT1 was involved in the glutamine metabolism of gastric cancer.The cell counting and Western blot detection showed that knocking down KCNQ1OT1 significantly reduced the gastric canc-er cell activity,SLC1A5 expression level and SLC1A5-mediated glutamine transport process(P＜0.01).Bioinformatics,RNA immunoprecipitation and dual luciferase analysis confirmed that KCNQ1OT1 com-petitively bind to miR-138-5p to promote the expression of SLC1A5.Finally,ChIP-seq data was used to detect the high H3K27ac signaling at the gene locus of KCNQ1OT1,and ChIP-qPCR was used to verify that P300-mediated enhancer activity regulated the high expression of KCNQ1OT1 in gastric cancer.KC-NQ1OT1 can serve as an independent diagnostic biomarker and prognostic predictor in various tumors.Targeting the KCNQ1OT1/miR-138-5p/SLC1A5 signaling axis to regulate glutamine metabolism may pro-vide new strategies and molecular targets for the treatment of gastric cancer.
