Mitochondrial Transcription Factor B1(TFB1M)Is Highly Expressed in Colon Cancer and Promotes Cell Growth Based on Bioinformatics Database
10.13865/j.cnki.cjbmb.2024.12.1303
- VernacularTitle:基于生物信息学分析线粒体转录因子B1在结肠癌中高表达并促进细胞生长
- Author:
Zhi-Gao OU
1
;
Hui-Ying CHEN
1
;
Ting TANG
1
;
Jian-Jun ZHU
1
Author Information
1. 山西医科大学基础医学院医学细胞生物与遗传学教研室,太原 030001
- Publication Type:Journal Article
- Keywords:
colon cancer(CC);
mitochondrial transcription factor B1(TFB1M);
cell viability;
cell ap-optosis
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(1):125-135
- CountryChina
- Language:Chinese
-
Abstract:
Mitochondrial transcription factor B1(TFB1M)is mainly involved in mitochondrial DNA transcription and related to the development of hepatocellular carcinoma and breast cancer.However,its role in colon cancer is unclear.In this study,the expression level of TFB1M in colon cancer and its prognosis were analyzed based on TCGA and other databases and IHC assays.Differentially expressed genes(DEGs)were screened and subjected to the analysis of functional enrichment,mutation analysis,immune cell infiltration,and drug sensitivity analysis.CCK-8 and flow cytometry were used to detect the effects of overexpression of TFB1M on the viability,apoptosis and cell cycle distribution of colon cancer cells.Our results showed that the expression level of TFB1M was significantly up-regulated in colon canc-er,and its expression level was correlated with the N stage and TNM stage(P＜0.05).The prognosis of colorectal cancer patients in the high TFB1M expression group was worse.Functional enrichment results showed that TFB1M was related to leukocyte-mediated immunity,immunoglobulin production and other signaling pathways.Mutation results showed that high-frequency mutated genes,such as ZFHX4,RYR2,PIK3CA and FAT4,had significantly higher mutation frequencies in the TFB1M high-expression group(all P＜0.05).In addition,the expression level of TFB1M was significantly higher in the PIK3CA and FAT4 mutation groups(all P＜0.05).Immune infiltration results showed that the percentage of CD4+memory activated T cells was increased in the TFB1M high expression group,while the percentage of Treg cells was reduced.The drug sensitivity results showed that patients in the TFB1M high expression group might be more sensitive to Tozasertib,cytarabine,vincristine,etc.,while patients in the TFB1M low expression group might be more sensitive to Dasatinib,JQ1,ERK_2440,etc.The results of cellular experiments showed that over-expression of TFB1M enhanced viability,reduced apoptosis and increased the percentage of S-phase and G2/M-phase cells in colon cancer cells.Altogether,the results indicated that TFB1M might play a key role in the growth of colon cancer cells by regulating immune cell infiltration and function.
