Exploring the Biogenesis Mechanism of β-Barrel Outer Membrane Proteins in Living Cells
10.13865/j.cnki.cjbmb.2024.12.1482
- VernacularTitle:β-桶外膜蛋白质生成机制的活细胞探究
- Author:
Yan WANG
1
;
Zeng-Yi CHANG
Author Information
1. 细胞逆境响应与代谢调控福建省高校重点实验室,福建师范大学生命科学学院,福州 350108
- Publication Type:Journal Article
- Keywords:
β-barrel outer membrane protein(OMP);
membrane protein biogenesis;
unnatural amino acid;
in vivo protein photo-crosslinking;
protein supercomplex
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(1):36-51
- CountryChina
- Language:Chinese
-
Abstract:
The β-barrel outer membrane protein is a kind of membrane integration protein widely existing in the outer membranes of Gram-negative bacteria,mitochondria and chloroplasts.The biogenesis of β-barrel outer membrane proteins in living bacterial cells is an extremely complex process,including the synthesis of nascent polypeptide chains in the cytoplasm,the translocation across the inner membrane,the transportation through the hydrophilic periplasmic space and finally the folding,assembly and inser-tion to the outer membrane to complete the whole process.During the biogenesis of β-barrel outer mem-brane proteins,it is critical to unveil how the periplasmic quality control factors such as SurA,Skp and DegP assist the folding and transportation of the nascent β-barrel outer membrane proteins through the hy-drophilic periplasmic space,and how the SecYEG-SecDF-Yidc complex on the inner membrane and the BAM complex on the outer membrane act during the biogenesis of β-barrel outer membrane proteins.This review delves into the research progress of the mechanism of β-barrel outer membrane protein biogenesis in Gram-negative bacteria and also the application of various new technologies such as in vivo protein pho-to-crosslinking analysis and high through-put protein electrophoresis(SDS-PAGE)for exploring such dy-namic cellular processes as protein biogenesis.
