The Xenomitochondrion Provides Extensive Supply for Mitochondrial Transplantation
10.13865/j.cnki.cjbmb.2025.01.1249
- VernacularTitle:异种线粒体可为线粒体移植提供来源
- Author:
Zhen YANG
1
;
Wen-Peng LI
;
Tian NIU
;
Hui-Wen XUE
;
Si-Xi ZHAO
;
Xing-Bo ZHAO
Author Information
1. 中国农业大学动物科学技术学院动物遗传育种与繁殖系,北京 100193
- Publication Type:Journal Article
- Keywords:
mitochondrial transplantation(MT);
xenomitochondria;
mtDNA copy number;
adenosine triphosphate(ATP);
reactive oxygen species(ROS)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(2):273-283
- CountryChina
- Language:Chinese
-
Abstract:
Mitochondria are organelles in eukaryotic cells that play a crucial role in cellular energy me-tabolism,oxidative stress,heat production,and signal transduction.Mitochondrial transplantation(MT)is currently one of the most advanced techniques for treating mitochondrial dysfunction and anti-aging re-search.This study aimed to explore the feasibility and effectiveness of xenogeneic MT by transplanting mitochondria from yak(Bos grunniens),domestic cattle(Bos taurus),and horse(Equus caballus)into mice(Mus musculus).The results demonstrated that mitochondria from yak,domestic cattle,and horse could be successfully transplanted into mice and maintained in various tissues and organs of the mice for at least 14 days,as confirmed by confocal imaging,digital PCR,and DNA sequencing.MT mice exhibi-ted positive biological effects,including increased ATP content and mitochondrial DNA copy number(P＜0.05),with the maximum effect observed on day 7,which was sustained until day 14.Reactive oxygen species(ROS)levels in MT mice significantly increased at 2 hours post-injection(P＜0.05),then grad-ually decreased towards baseline levels by day 7 and day 14(P＞0.05).These findings support the effec-tiveness of xenogeneic MT and suggest that the effects can be maintained for up to 14 days.This study provides scientific evidence for future clinical applications.
