An Epithelial Senescence Model Induced by Doxorubicin in MCF 10A Cells
10.13865/j.cnki.cjbmb.2024.11.1342
- VernacularTitle:阿霉素诱导MCF 10A上皮细胞衰老模型的构建
- Author:
Zeng-Sheng WANG
1
;
Zu-Biao NIU
;
Bo ZHANG
;
Jia-Hui HAO
;
Yi-Chao ZHU
;
Rui-Gang YANG
;
He REN
;
Chen-Yu LIU
;
Qiang SUN
;
Li-Cheng REN
Author Information
1. 海南医科大学基础医学与生命科学学院热带转化医学教育部重点实验室,生物学教研室,海口 571199;军事科学院军事医学研究院前沿生物技术实验室,北京 100071
- Publication Type:Journal Article
- Keywords:
epithelial cell senescence model;
doxorubicin;
MCF 10A;
β-galactosidase(SA-β-gal);
senescence-associated secretory phenotype(SASP)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(1):147-155
- CountryChina
- Language:Chinese
-
Abstract:
This research aims to construct a stable epithelial cell senescence model for screening and e-valuation of senolytics.We explored the optimal conditions for doxorubicin-induced senescence of non-transformed epithelial cells MCF 10A,including the optimal induction concentration,the optimal inter-vention time,and the optimal senescence duration,and confirmed the feasibility of MCF 10A as an epi-thelial senescence model by multiple ways.The optimal condition for Doxorubicin-induced senescence of MCF 10A cells was treatment with 0.6 μmol/L Doxorubicin for 16 h to achieve the best senescence state on the 8th day.Under the optimal induction conditions,the positive rate of senescence-associated β-gal-actosidase(SA-β-gal)staining in the treated group reached 97％.At the same time,biochemical results of detecting the expression of mRNA,proteins,and immunofluorescence demonstrated that the expression levels of senescence-associated secretory phenotype(SASP),p16,p21 and p53 in the treated group were significantly higher than those in the control cells,and Lamin B1 was significantly decreased(P＜0.001),which were consistent with the specific characteristics of senescence.In summary,an epithelial senescence model was successfully induced in MCF 10A cells by Doxorubicin in this study,which will promote the screening of senolytics for senescent epithelial cells.
