Resistance Exercise Activates Piezo1/AMPK/PGC-1α,Ameliorating Disuse-Induced Skeletal Muscle Atrophy in Mice
10.13865/j.cnki.cjbmb.2024.12.1407
- VernacularTitle:抗阻运动激活Piezo1/AMPK/PGC-1α缓解小鼠废用性骨骼肌萎缩
- Author:
Dong-Sheng HAN
1
;
Qing-Yu LIANG
;
Xiao-Feng SHI
Author Information
1. 山西大学体育学院,太原 030006
- Publication Type:Journal Article
- Keywords:
resistance exercise;
piezoelectric mechanosensitive ion channel component 1(piezo 1);
dis-use-induced skeletal muscle atrophy;
myogenesis
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(1):136-146
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate whether resistance exercise can effectively alleviate disuse skel-etal muscle atrophy by activating the skeletal muscle Piezo1/AMPK/PGC-1α signaling pathway,impro-ving mitochondrial function and promoting myogenesis.8-week-old male C57BL/6J mice were used.Dis-use muscle atrophy was simulated by hind limb plaster fixation,and the mice were divided into the con-trol group,the disuse muscle atrophy group,the disuse muscle atrophy+resistance exercise group,and the disuse muscle atrophy+resistance exercise+Piezo1 inhibitor group.The resistance exercise group underwent an 8-week resistance training program.Methods such as Western Blotting,immunofluores-cence staining,and mitochondrial mass and function detection were employed to evaluate skeletal muscle mass,function,mitochondrial status,and myogenesis.Resistance exercise significantly increased the cross-sectional area of skeletal muscle in mice with disuse muscle atrophy by 23％(P＜0.01)and the relative mass of skeletal muscle by 11％(P＜0.01).Resistance exercise led to an average increase of 206 m in the longest running distance of mice(P＜0.01),an average increase of 36.7 g in the maxi-mum bearing capacity(P＜0.01),and an average reduction of 1.5 s in the balance beam crawling time(P＜0.01).Meanwhile,it upregulated the expression of the protein levels of Piezo1,AMPK,and PGC-1α(P＜0.01).In addition,resistance exercise increased the activity or protein expression levels of MMP,TFAM,COX Ⅰ,CS,ATPB,ATPase,ATP,p-mTOR/mTOR,p-P70S6K/P70S6K,Pax7,and MyoD in skeletal muscle(P＜0.05,P＜0.01).However,inhibiting Piezo1 decreased the expres-sion of the above enzyme activities and protein levels(P＜0.05,P＜0.01).Resistance exercise allevi-ates disuse skeletal muscle atrophy effectively by activating the Piezo1/AMPK/PGC-1α pathway,impro-ving skeletal muscle mitochondrial quality and function,and promoting protein synthesis and myogenesis.
