The comparison of ferroptosis characteristics and motor deficits in Parkinson's disease mouse models
10.3969/j.issn.1006-5725.2025.22.006
- VernacularTitle:帕金森病小鼠动物模型铁死亡特征及运动障碍差异比较
- Author:
Haiyan LI
1
;
Mengzhu LI
;
Mengxuan CHEN
;
Da GAO
;
Kexin DUAN
;
Lijun ZHAO
;
Meiling ZHU
Author Information
1. 广州中医药大学第四临床医学院(广东 深圳 518033);深圳市中医院医学检验科(广东 深圳 518033)
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
ferroptosis;
6-OHDA;
MPTP;
LPS;
animal model
- From:
The Journal of Practical Medicine
2025;41(22):3501-3509
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare ferroptosis characteristics and motor dysfunction across different mouse models of Parkinson's disease(PD).Methods Animal models of PD were divided into five groups:the control group,sham-operated group,6-hydroxydopamine(6-OHDA)group,1-methyl-4-phenyl-1,2,3,6-tetrahydropyri-dine(MPTP)group,and lipopolysaccharide(LPS)group.Differences in ferroptosis-related features and motor dysfunction across these groups were evaluated through behavioral observation,histopathological examination,protein analysis,and assessment of lipid peroxidation and oxidative stress levels.Results In all three model groups,the number of TH-positive neurons in the substantia nigra on the lesioned side was significantly reduced,accompanied by evident neuronal degeneration and a marked decrease in Nissl bodies.Behavioral assessments revealed that the 6-OHDA group displayed the most severe motor deficits.In the substantia nigra,FTH1 protein expression was significantly downregulated in the 6-OHDA,LPS,and MPTP groups(P<0.01,P<0.05,P<0.01),respectively,while GPX4 expression was also reduced(P<0.001,P<0.001,P<0.01).Malondialdehyde(MDA)levels were significantly elevated in both the 6-OHDA and MPTP groups(P<0.05,P<0.05).Glutathione(GSH)assays demonstrated markedly reduced levels in the 6-OHDA,MPTP,and LPS groups(P<0.01,P<0.001,P<0.001).Reactive oxygen species(ROS)detection revealed a significant increase in the MPTP and LPS groups(P<0.05,P<0.05).Conclusion The 6-OHDA model is well suited for studying PD-related behaviors and the underlying mechanisms of motor symptoms,the MPTP model is particularly effective for investigating the ferroptosis pathway,and the LPS model serves as a valuable complement for research on neuroinflammation mechanisms.
