Revealing potential mechanism of Danggui Buxue decoction in regulating immuno-suppression via PI3K-AKT signaling pathway using network pharmacology and in vivo experiment
10.16303/j.cnki.1005-4545.2025.02.23
- VernacularTitle:基于网络药理学和体内试验揭示当归补血汤通过PI3K-AKT信号通路调节免疫抑制的潜在机制
- Author:
Huan HUANG
1
;
Pingrui YANG
;
Xifeng LI
;
Fuxing GUI
;
Yutao LI
;
Li ZHANG
;
Shuo LIU
;
Yufei XIE
;
Hongxu DU
;
Shicheng BI
;
Liting CAO
Author Information
1. 西南大学动物医学院,重庆荣昌 402460
- Publication Type:Journal Article
- Keywords:
Danggui Buxue decoction;
immunosuppression;
network pharmacology
- From:
Chinese Journal of Veterinary Science
2025;45(2):350-361
- CountryChina
- Language:Chinese
-
Abstract:
Danggui Buxue decoction(DBD)is a classic prescription with immunomodulatory and hematopoietic effects.Previous studies have shown the DBD has potential to be used as an oral im-mune booster.However,its immunomodulatory effects and mechanism of action have not been thoroughly studied,especially the protective mechanism of immunomodulatory regulation in the state of immunosuppressive is still unclear.The aim of this study was to explore the protective mechanism of DBD in the immunosuppressive state using network pharmacology combined with animal experiments verification.The active components,core targets and signaling pathways of DBD in treating immunosuppression were obtained using network pharmacology tools.On this ba-sis,the active components of DBD were identified using HPLC-MS,and in vivo studies were con-ducted at the same time.The key active components of DBD obtained using network pharmacology included quercetin,kaempferol and formononetin.The core targets included TP53,RELA,TNF,AKT1,and IL-6.KEGG pathway enrichment analysis showed that phosphoinositide 3-kinase(PI3K)-protein kinase B(AKT)may play an important role in the treatment of immunosuppres-sive diseases using DBD.Molecular docking confirmed that each core target had good binding activ-ity with its corresponding compounds.Animal experiments showed that after DBD intervention,the mRNA gene and protein expression of RELA,TNF,and IL-6 in the serum was significantly down-regulated.The mRNA expression of PI3K and AKT in the ileum and PI3K protein expression were also downregulated.In conclusion,DBD exerts its role in treating immunosuppressive diseases by regulating the PI3K-AKT signaling pathway.
