Research on mechanism of apigenin against transmissible gastroenteritis virus in-fection based on network pharmacology and molecular docking
10.16303/j.cnki.1005-4545.2025.02.19
- VernacularTitle:基于网络药理学和分子对接技术研究芹黄素抗猪传染性胃肠炎病毒的作用
- Author:
Yi ZHANG
1
;
Yuxin TANG
;
Chenxi SHI
;
Hui HU
Author Information
1. 河南农业大学动物医学院,河南郑州 450046
- Publication Type:Journal Article
- Keywords:
network pharmacology;
apigenin;
transmissible gastroenteritis virus;
antiviral infection
- From:
Chinese Journal of Veterinary Science
2025;45(2):312-321
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to explore the mechanism of apigenin against transmissible gas-troenteritis virus(TGEV)infection based on network pharmacology and molecular docking.The potential targets of apigenin were obtained from Pharmmapper,Pubchem and other databases.The PubMed database was searched to obtain the relevant targets of TGEV infection.The intersection targets of apigenin and TGEV infection were identified by Draw Venn Diagram online program.A"drug-disease-target"network was constructed using STRING database and Cytoscape 3.8.2 soft-ware.Protein-protein interaction relationships were obtained from the STRING database,and core targets were analyzed.The intersection targets were subjected to GO function and KEGG pathway enrichment analysis using the DAVID database.Finally,the analysis results were validated through molecular docking and in vitro cell experiments.The study identified 431 targets for apigenin,1 177 targets for TGEV infection,and 50 intersection targets for apigenin and TGEV infection.GO enrichment analysis indicated that apigenin was mainly involved in regulating cell differentiation,cell membrane raft formation,apoptosis,and inflammatory responses.The top 15 statistically sig-nificant KEGG enrichment results mainly involve the PI3K-Akt signaling pathway and TNF signa-ling pathway.Docking analysis showed that apigenin had the strongest interaction with matrix metalloproteinase 3(MMP3)with an affinity of-9.5 kJ/mol and the binding activity of MMP3 was the best.The results of in vitro experiments demonstrated that treatment of different concen-trations of apigenin significantly reduced virus titers,virus genome copies,and the expression lev-els of MMP3 and its upstream and downstream proteins compared to the virus-infected group.Api-genin may exert its anti-TGEV effects through multiple targets and pathways,possibly by regula-ting the NF-κB-MMP3-IL-1β signaling pathway.
