Dynamic changes of serum cytokines in mice following gastrointestinal infection with porcine-derived Proteus mirabilis
10.16303/j.cnki.1005-4545.2025.02.15
- VernacularTitle:猪源奇异变形杆菌感染胃肠道后小鼠血清中细胞因子的动态变化
- Author:
Jie ZHOU
1
;
Wengui FU
;
Chengsen HAO
;
Shaomei LI
;
Shaoqin ZHAI
;
Rui YANG
Author Information
1. 重庆市畜牧科学院,重庆荣昌 402460;国家生猪技术创新中心(重庆),重庆荣昌 402460
- Publication Type:Journal Article
- Keywords:
Proteus mirabiliss;
cytokines;
spleen;
mice
- From:
Chinese Journal of Veterinary Science
2025;45(2):281-288
- CountryChina
- Language:Chinese
-
Abstract:
Proteus mirabilis is a significant foodborne pathogen that frequently causes diseases in humans and animals.Understanding the cytokine profile alterations following gastrointestinal in-fection with P.mirabilis is crucial for developing effective treatment strategies.This study em-ployed an intragastric infection model in mice to observe clinical symptoms and pathological chan-ges.Blood samples were collected at various time points post-infection,and the concentrations of 21 cytokines in the serum of both infected and control mice were quantitatively analyzed using the Bio-Plex suspension array system.The results of the infection experiment indicated that gastroin-testinal infection with P.mirabilis in mice led to splenic hemorrhage and significant splenomegaly.A notable difference in spleen index between the infected and control groups was observed at 24 hours post-infection(P=0.001 688),with the greatest difference occurring at 96 hours post-infec-tion(P=0.000 074).Cytokine analysis revealed significant elevations in IL-2,IL-17A,KC,Eotax-in,MCP-1,RANTES,MIP-1α,MIP-1β,IFN-γ,G-CSF,and IL-10 levels in the infected group.A-mong these,the pro-inflammatory cytokines IL-17A and G-CSF exhibited the most substantial changes,positively correlating with the splenomegaly,and peaking at 96 hours post-infection,with concentrations of(104.74±3.91)and(5 184.08±280.22)ng/L,respectively,which were 26.79 and 36.25 times higher than those in the control group.These findings indicate that IL-17A and G-CSF play significant roles in the pro-inflammatory response following gastrointestinal infection with P.mirabilis.
