Construction and expression of an ETEC adhesin multi-epitope fusion antigen and evaluation of its immunogenicity
10.16303/j.cnki.1005-4545.2025.02.08
- VernacularTitle:一种ETEC黏附素多表位融合抗原的构建与表达及其免疫原性评价
- Author:
Yayun YANG
1
;
Huabo SUN
1
;
Jiashu CHANG
1
;
Jinxin HE
1
;
Shaopeng GU
1
Author Information
1. 山西农业大学 动物医学学院,山西 晋中 030801
- Publication Type:Journal Article
- Keywords:
enterotoxigenic Escherichia coli;
adhesin;
multiepitope fusion antigen;
adhesion inhibi-tion
- From:
Chinese Journal of Veterinary Science
2025;45(2):227-234,242
- CountryChina
- Language:Chinese
-
Abstract:
Enterotoxigenic Escherichia coli(ETEC)is the main cause of diarrhea in piglets,which seriously threatens the development of animal husbandry.In order to effectively prevent and con-trol piglet diarrhea,the neutralizing epitopes of the screened K88(FTDYEGASVELRKPDGGT-NK),K99(NVGNGSGGANIN),987P(LAAPAENNTSQAN),F41(VMAADWTEGQPGDII)and F18(PPNAQTYPLSSGDLK)ETEC adhesins were assembled to construct the multi-epitope fusion antigen(MEFA),whose immunogenicity was verified by in silico simulations and in vitro.The computer simulation results showed that MEFA has excellent physicochemical properties and reasonable prediction results of secondary and tertiary structures and exhibits strong interaction force when docking with toll-like receptor 3,and the kinetic simulation shows that it can be stably presented.In vitro validation results showed that anti-K88,K99,987P,F18 and F41 IgY could spe-cifically recognize MEFA proteins.After immunization of laying hens with MEFA,the titers of IgY antibodies against the five ETEC were higher than those of non-specific IgY.In addition,specific anti-MEFA IgY significantly inhibited the adhesion of K88,K99,987P,F41 and F18 ETEC strains to piglet intestinal IPEC-J2 cell lines.These results indicate that MEFA has strong immunogenicity and can be used as an ideal vaccine candidate for the prevention of K88,K99,987P,F41 and F18 ETEC infection,and provide reference and technical support for the development of multivalent broad-spectrum ETEC vaccine candidates.
