Isolation,identification and antimicrobial susceptibility of a strain of Haemophi-lus parasuis
10.16303/j.cnki.1005-4545.2025.02.07
- VernacularTitle:1株副猪嗜血杆菌分离鉴定及耐药性分析
- Author:
Xi LIU
1
;
Geng WANG
;
Zhengdan LIN
;
Xiuxiu SUN
;
Xinxin JIN
;
Li LI
;
Junjie YANG
;
Xue-ying HU
;
Changqin GU
;
Wanpo ZHANG
;
Xiaoli LIU
;
Teng YU
;
Guofu CHENG
Author Information
1. 华中农业大学动物医学院,湖北武汉 430070
- Publication Type:Journal Article
- Keywords:
arthritis;
HPS;
PCR identification;
pig
- From:
Chinese Journal of Veterinary Science
2025;45(2):219-226
- CountryChina
- Language:Chinese
-
Abstract:
Porcine arthritis,one of the common chronic diseases in large-scale pig farms,can signifi-cantly reduce the production performance of meat pigs.In this study,a strain of Haemophilus pa-rasuis(HPS)was isolated from the joint fluid of a lame pig.The HPS was analyzed in terms of se-rotypes,virulence genes,and resistance genes.Additionally,it was treated with sensitive antibiotics to provide a theoretical basis for the comprehensive prevention and treatment of arthritis in meat pigs in future production settings.A strain of HPS type 14 was isolated from the joint fluid of dis-eased pigs.The HPS isolate demonstrated sensitivity to β-lactams and tetracyclines,while florfeni-col and polymyxin effectively inhibited its growth at low concentrations.However,the bacteria ex-hibited resistance to sulfonamides and ciprofloxacin.The treatment of affected pigs with clinical ar-thritis using doxycycline and enrofloxacin injections proved effective.Compared to the infected group,in which the sick pigs experienced difficulty flexing their carpal and tarsal joints and exhibi-ted significant lameness,the pigs in the treatment group showed marked improvement.Their joints were only slightly swollen,and the clinical symptoms of arthropathy were alleviated.
