Effect of aflatoxin B1 on replication of porcine delta coronavirus infection
10.16303/j.cnki.1005-4545.2025.02.03
- VernacularTitle:黄曲霉毒素B1对猪δ冠状病毒感染复制的影响
- Author:
Yifan FENG
1
;
Qinghao LI
1
;
Manxi WANG
1
;
Yuqing TANG
1
;
Ming LI
1
;
Qian-qian GUO
1
;
Juan SUN
1
;
Yilei LI
1
;
Xin JIN
1
Author Information
1. 河南农业大学动物医学院 河南郑州 450046;动物病原与生物安全教育部重点实验室,河南郑州 450046;河南省动物性食品病原监测实验室,河南郑州 450046
- Publication Type:Journal Article
- Keywords:
aflatoxin b1;
porcine delta coronavirus;
replication effect
- From:
Chinese Journal of Veterinary Science
2025;45(2):187-194
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the effects of aflatoxin B1(AFB1)on the infection and replication of porcine delta coronavirus(PDCoV).Porcine small intestinal epithelial cells(IPEC-J2),porcine kidney cells(LLC-PK)and swine testis cells(ST)were used as models,and CCK-8 method was used to detect the safe mass concentration range of AFB1 on the three cell lines.Each cell line was divided into the blank control group,AFB1 treatment group,PDCoV treatment group and AFB1 and the PDCoV co-treatment group.The cells were treated with safe mass concentration of AFB1 for 12 h,and then treated with PDCoV for 20 h.Total RNA and total protein were extrac-ted from the cells.The effects of AFB1 on PDCoV infection and replication were detected by qPCR,Western blot and cell immunofluorescence tests.The results showed that compared with the PDCoV treatment group,the mRNA and N protein of viral S protein in the AFB1 and PDCoV co-treatment group were significantly increased(P＜0.05),and a significantly higher fluorescence of PDCoV N protein is also visibly present in the co-treatment group,and there was a significant difference between the two groups.The results showed that AFB1 could promote the infection and replication of PDCoV.It provides important data that AFB1 can promote the infection replication of PDCoV and provides a new idea for the prevention and treatment of PDCoV.
