Establishment and preliminary application of quadruple qPCR method for PRV,PPV,PCV2 and ASFV
- VernacularTitle:PRV、PPV、PCV2和ASFV四重qPCR检测方法的建立及初步应用
- Author:
Xu CHEN
1
;
Deyuan TANG
1
;
Zhiyong ZENG
1
;
Bin WANG
1
;
Shenglin YUAN
1
;
Zhengbo LIAO
1
;
Song HE
1
;
Piao ZHOU
1
;
Yinming MAO
1
Author Information
- Publication Type:Journal Article
- Keywords: porcine pseudorabies virus; porcine parvovirus; porcine circovirus; African swine fever virus; quadruple PCR
- From: Chinese Journal of Veterinary Science 2025;45(2):175-180,194
- CountryChina
- Language:Chinese
- Abstract: To identify clinical viral diseases characterized by reproductive disorders and abortion,a quadruple qPCR method was established for simultaneous detection of PRV,PPV,PCV2 and AS-FV.Four pairs of specific primers and probes were designed according to the conserved genes of four viruses in the NCBI gene bank.The annealing temperature,primer concentration and probe concentration of the reaction were optimized,and the specificity,sensitivity and repeatability of the method were tested.The results showed that the method could not detect other pathogens except the target ones.The minimum detection limit of PRV,PPV,PCV2 and ASFV was 10 copies.Intra-group and inter-group repeatability tests showed that the coefficient of variation of C,values be-tween different batches was less than 3％,indicating that the method was highly specific,sensitive and stable.Establishment of an efficient and sensitive quadruple qPCR method provides technical reference for the clinical prevention and control of porcine pseudorabies virus disease,porcine circo-virus disease,porcine parvovirus disease and African swine fever.
