Expression and Clinical Significance of Serum miR-182-5p and miR-142-5p in Patients With Ulcerative Colitis
10.3969/j.issn.1008-7125.2024.11.004
- VernacularTitle:血清miR-182-5p和miR-142-5p在溃疡性结肠炎患者中的表达及其临床意义
- Author:
Hongmin YIN
1
;
Shuijin ZHU
1
;
Min HUANG
1
;
Jun WANG
1
Author Information
1. 上海交通大学医学院附属新华医院崇明分院消化内科(202150)
- Publication Type:Journal Article
- Keywords:
Colitis,Ulcerative;
miR-182-5p;
miR-142-5p;
Diagnosis
- From:
Chinese Journal of Gastroenterology
2024;29(11):662-667
- CountryChina
- Language:Chinese
-
Abstract:
Background:There is no gold standard for the diagnosis of ulcerative colitis(UC).Given that microRNAs(miRNAs)have been shown to play an important role in inflammation,cell death,and cancer progression,detection of relevant serum markers may be valuable in the diagnosis of UC.Aims:To analyze the expression and clinical significance of miR-182-5p and miR-142-5p in the serum of patients with UC.Methods:From May 2021 to May 2023,86 UC patients admitted to our hospital were collected.According to the clinical severity,UC patients were grouped into mild group,moderate group,and severe group.86 healthy volunteers(control group)who underwent physical examinations in our hospital were collected.qRT-PCR was applied to detect serum levels of miR-182-5p and miR-142-5p.Spearman method was applied to analyze the relationship between the expression levels of miR-182-5p and miR-142-5p in the serum of UC patients and the Mayo score;;Pearson method was applied to analyze the relationship between the expression levels of miR-182-5p and miR-142-5p in serum of UC patients and the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and IL-6.ROC curve was applied to analyze the diagnostic value of serum miR-182-5p and miR-142-5p for UC.Decision curve analysis(DCA)was used to determine the clinical practicability of serum miR-182-5p and miR-142-5p and combined detection in the diagnosis of UC.Results:Compared with the control group,the expression levels of serum miR-182-5p,TNF-α,IL-1β,and IL-6 in the UC group were greatly increased,while the expression level of miR-142-5p was greatly reduced(P＜0.05),there was no great difference in IL-10 level between the two groups(P＞0.05).Correlation analysis showed that the expression level of serum miR-182-5p was positively correlated with TNF-α,IL-1β,and IL-6 levels,while the expression level of serum miR-142-5p was negatively correlated with TNF-α,IL-1β,and IL-6 levels(P＜0.05).Compared with the mild group,the expression level of miR-182-5p and Mayo score in the serum of UC patients in the moderate and severe groups increased greatly,while the expression level of miR-142-5p decreased greatly(P＜0.05).Correlation analysis showed that the expression level of serum miR-182-5p was positively correlated with the Mayo score(rs=0.774,P＜0.001);the expression level of serum miR-142-5p was negatively correlated with Mayo score(rs=-0.523,P＜0.001).The AUC value of miR-182-5p combined miR-142-5p in diagnosing UC was higher than that of miR-182-5p and miR-142-5p alone(P＜0.05).The results of DCA curve showed that when the high risk threshold was 0.08-0.95,the net benefit rate of the combined detection of serum miR-182-5p and miR-142-5p in the diagnosis of UC was better than that of miR-182-5p and miR-142-5p alone.Conclusions:The expression level of miR-182-5p in serum of UC patients was significantly increased,and the expression level of miR-142-5p was significantly decreased,both of which could be used as indicators for observing the disease activity of UC.
