Correlation between lncRNA LOC101927476, SSEA-4, hsa-miR-28 and postoperative recurrence/metastasis of ovarian cancer
10.3760/cma.j.cn115807-20241122-00366
- VernacularTitle:LncRNA LOC101927476、SSEA-4、hsa-miR-28与卵巢癌术后复发/转移相关性探究
- Author:
Xia ZHANG
1
;
Aiqin NIU
;
Fei LI
;
Xia LI
Author Information
1. 商丘市第一人民医院妇科，商丘 476100
- Publication Type:Journal Article
- Keywords:
LncRNA LOC101927476;
SSEA-4;
hsa-miR-28;
Ovarian cancer;
Recurrence;
Metastasis
- From:
Chinese Journal of Endocrine Surgery
2025;19(4):595-600
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the correlation between long non-coding RNA LOC101927476 (LncRNA LOC101927476), stage-specific embryonic antigen-4 (SSEA-4), and intronic microRNA-28 (hsa-miR-28) and postoperative recurrence/metastasis of ovarian cancer.Methods:A total of 195 patients with ovarian cancer who underwent surgical treatment in The First People’s Hospital of Shangqiu and The First Affiliated Hospital of Zhengzhou University from Jan. 2021 to Oct. 2022 were selected. Patients were divided into occurrence group and non-occurrence group according to whether they had recurrence/metastasis within 2 years after surgery. R package "Match It" and the 1∶1 principle for propensity score matching (PSM) were used to compared the expression of LncRNA LOC101927476, SSEA-4 mRNA, and hsa-miR-28 in different tissues and two groups. Multivariate logistic regression analysis was used to analyze the correlation between various detection indicators in cancer tissues and postoperative recurrence/metastasis of ovarian cancer. The value of LncRNA LOC101927476, SSEA-4 mRNA, hsa-miR-28, and their combined use in predicting recurrence/metastasis in cancer tissues was analyzed using the receiver operating characteristic (ROC) curve. The external calibration curve was used to analyze the combined predicts of the consistency between the incidence of recurrence/metastasis and the actual incidence.Results:In cancer tissues, the expression of LncRNA LOC101927476 and hsa-miR-28 was lower than that in adjacent tissues, while the expression of SSEA-4 mRNA was higher ( P<0.05). The expression of LncRNA LOC101927476 and hsa-miR-28 in the occurrence group was lower than that in the non-occurrence group, while the expression of SSEA-4 mRNA was higher than that in the non-occurrence group ( P<0.05). Multivariate logistic regression analysis showed that the increase of LncRNA LOC101927476, SSEA-4 mRNA, and hsa-miR-28 were independent factors associated with the recurrence/metastasis of ovarian cancer after surgery ( P<0.05). ROC analysis showed that the AUCs of LncRNA LOC101927476, SSEA-4 mRNA, hsa-miR-28, and their combined prediction of ovarian cancer recurrence/metastasis after surgery were 0.730, 0.767, 0.832, and 0.915, respectively ( P<0.001). Comparing the AUC of the combination with that of the individual, it was found that the AUC of the combination was significantly higher than that of LncRNA LOC101927476, SSEA-4 mRNA, and hsa-miR-28 ( Z=3.924, 2.995, 2.078, P=0.000, 0.003, 0.038). The calibration curve of the external dataset showed that the combined prediction of the incidence of recurrence/metastasis was basically consistent with the actual incidence, and the two curves had a high degree of fit. Conclusions:The expression of LncRNA LOC101927476, SSEA-4, and hsa-miR-28 in cancer tissues is associated with postoperative recurrence/metastasis of ovarian cancer, which can provide a reference for early clinical prediction of recurrence/metastasis. The combined application of the three can further improve the predictive value, help to early warn the risk of recurrence/metastasis, and provide important reference information for clinical personalized prevention intervention.
