Mechanism of doxorubicin/copper complex induced cuproptosis in hepatocellular carcinoma cells
10.12092/j.issn.1009-2501.2025.01.001
- VernacularTitle:阿霉素/铜复合物诱导肝癌细胞发生铜死亡的机制研究
- Author:
Jing LIU
1
;
Guojie LEI
;
Jinghao CAO
;
Lingyan YU
;
Jing DU
;
Ying WANG
Author Information
1. 锦州医科大学研究生培养基地浙江省人民医院,杭州 310014,浙江;浙江省人民医院(附属人民医院)检验中心,杭州医学院,杭州 310014,浙江
- Publication Type:Journal Article
- Keywords:
doxorubicin;
copper;
hepatocellular carcinoma;
cuproptosis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(1):1-10
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the mechanism of doxorubicin/copper(DOX/Cu)complex induced copper death in hepatocellular carcinoma cells.METHODS:Human hepatocellular carcinoma cell line Huh7 was treated with DOX/Cu 0,2.5,4,7.5,10 and 12.5 μmol/L.The cell viability was detected by CCK-8 method.The cell proliferation level was observed by laser microscopy and proliferation kit.The cell invasion ability was determined by cell scratch assay.The flow cytometry was used to de-tect intracellular reactive oxygen species(ROS)and copper ion levels.And the western blot was used to detect intracellular iron-sulfur cluster proteins expression levels.RESULTS:With the increase of DOX/Cu concentration,cell viability,cell prolifera-tion and invasion ability decreased gradually.The copper ion chelating agent(TTM)can significantly restore the effects of DOX/Cu on cell viability.After DOX/Cu treatment,intracellular copper ion and ROS levels related to coproptosis were significantly increased,accompanied by the loss of iron-sulfur cluster proteins.CONCLUSION:DOX/Cu can inhibit hepatocellular carcinoma cells through cuproptosis.
