Study on Chemical Identification of Bupleurum Chinense and Counterfeits Based on Characteristic Chromatogram and UP-LC-Q-TOF/MS Technology
10.14148/j.issn.1672-0482.2025.0066
- VernacularTitle:基于特征图谱和UPLC-Q-TOF/MS技术的北柴胡及其常见混淆品的化学鉴别研究
- Author:
Yanyan GUO
1
;
Huanhuan WANG
1
;
Ziyi ZHONG
1
;
Xinlian LIU
1
;
Li SUN
1
;
Jing WANG
1
Author Information
1. 南京中医药大学附属中西医结合医院,江苏 南京 210028;江苏省中医药研究院 国家中医药管理局中药口服制剂释药系统重点研究室,江苏 南京 210028
- Publication Type:Journal Article
- Keywords:
Bupleurum;
characteristic chromatogram;
UPLC-Q-TOF/MS;
TLC;
chemical component;
authenticity identification
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(1):66-77
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To identify Bupleurum chinense pieces and its counterfeits based on the characteristic chromatogram and UPLC-Q-TOF/MS technology.METHODS Thin layer chromatography was used to identify Bupleurum chinense and its counterfeits collected from different origins and different producing areas.Then,the chemical constituents of Bupleurum chinense and its counter-feits were compared according to the established HPLC characteristic chromatogram,and the representative differential markers of Bup-leurum chinense counterfeits were further identified by UPLC-Q-TOF/MS technology.RESULTS Thin layer chromatography showed that different original Bupleurum chinense pieces had saikosaponin A and saikosaponin D,which could be distinguished according to the intensity and position of fluorescent spots.There were 14 and 16 common peaks in the specific chromatogram of Bupleurum chinense and its vinegar-processed products respectively,and 6 components were identified.The chemical components of Bupleurum chinense pieces from different producing areas were similar,and the established method could better reflect the characteristics of Bupleurum chinense.Further comparison of the specific chromatogram of Bupleurum chinense and its counterfeits showed that the composition of B.marginatum Wall.was close to that of the authentic Bupleurum chinense.,B.bicaule Helm and B.longiradiatum Turcz.had their own characteristic peaks with high response values.The content of saponins in B.marginatum var.stenophyllum was significantly high-er.A total of 69 Bupleurum compounds were identified by UPLC-Q-TOF/MS mainly triterpenoid saponins,followed by flavonoids,a few chromones,phenylpropanoids and alkynes.The results of cluster analysis showed that the interspecific differences of Bupleurum chinense were obvious,and different Bupleurum counterfeits had representative differential markers.CONCLUSION The established characteristic chromatogram and UPLC-Q-TOF/MS method can be used for the chemical identification of Bupleurum chinense and its counterfeits,which provide a basis for the comprehensive and rational development and utilization of Bupleurum resources.
