Nrf1 attenuates neuronal injury caused by oxygen glucose depriva-tion/reperfusion via inhibiting apoptosis
10.12092/j.issn.1009-2501.2025.01.002
- VernacularTitle:Nrf1通过抑制细胞凋亡减轻氧糖剥夺/复糖复氧致神经元损伤
- Author:
Rongsong XIA
1
;
Jing YANG
;
Hong WANG
;
Zhe PENG
;
Yibei ZHAO
;
Junqing YANG
Author Information
1. 重庆医科大学药理学系生物化学与分子药理学重点实验室,重庆 400016;重庆市人民医院药学部,重庆 401147
- Publication Type:Journal Article
- Keywords:
OGD/R;
Nrf1;
Bax;
bcl-2;
apoptosis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(1):11-19
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of Nrf1 on neuronal apoptosis treated by oxygen-glucose deprivation/reperfusion and the mechanism.METHODS:Single-cell sequencing data was ana-lyzed by GEO database,and the correlation of Nrf1 expression with apoptotic pathways evaluated based on GSVA package calculations.PC12 cells and primary neurons were divided into the Normal group,the OGD/R group,the OGD/R+siRNA-NC group,and the OGD/R+Nrf1-siRNA-2 group.Cell images was observed by laser scanning confocal mi-croscopy;the viability of cells were detected by MTT assay;the apoptosis of cells were detected by flow cytometry;DHE fluorescent probe to detect ROS levels,the protein expression of Nrf1,bcl-2 and Bax were detected by Western blot;and nuclear translocation was observed by laser scanning confo-cal microscope.RESULTS:The results of biosigna-ture analysis revealed that Nrf1 was mainly en-riched in the apoptotic pathway;compared with normal group,the cell body became smaller,syn-apse broke,cells clustered in PC12 cells and prima-ry neurons with OGD/R treated,and the vitality of PC12 cells and neuronal were decreased significant-ly(P＜0.01);ROS levels were significantly higher(P＜0.01),the expressions of Nrf1 and Bax were in-creased significantly,and the expression of bcl-2 was decreased significantly(P＜0.05).Compared with the OGD/R group,there was no significant dif-ference in the siRNA-NC group;compared with the siRNA-NC group,the viability of cells was de-creased significantly(P＜0.01);ROS levels were sig-nificantly increased(P＜0.01),the expressions of Nrf1 and Bax were increased markedly,and the ex-pressions of bcl-2 was decreased significantly(P＜0.05)in Nrf1-siRNA-2 group.CONCLUSION:Nrf1 at-tenuates neuronal injury caused by oxygen glucose deprivation/reperfusion via inhibiting apoptosis.
